Abstract

We are engaged in recording the properties of ionic channels that are activated by chemical means, particularly post synaptic receptors, in tissue cultured nerve and muscle. Using fluctuation analysis to estimate single channel conductance and channel open time for a variety of channels activated by different drugs, we will also measure voltage sensitivity if possible using fluctuation techniques and voltage clamp step procedures. We will look at how different pharmacological agents, particularly central nervous system active drugs, modify post synpatic channel function. We will also attempt to record the opening and closing of single channels in order to observe channel transitions in a theory free manner.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
9R01DK037792-09A1
Application #
3236807
Study Section
Physiology Study Section (PHY)
Project Start
1977-09-01
Project End
1991-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
State University of New York at Buffalo
Department
Type
Schools of Medicine
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260

  Publications

Ding, J P; Pickard, B G (1993) Mechanosensory calcium-selective cation channels in epidermal cells. Plant J 3:83-110
Ruknudin, A; Sachs, F; Bustamante, J O (1993) Stretch-activated ion channels in tissue-cultured chick heart. Am J Physiol 264:H960-72
Ding, J P; Pickard, B G (1993) Mechanosensory calcium-selective cation channels in epidermal cells. Plant J 3:83-110
Bowman, C L; Ding, J P; Sachs, F et al. (1992) Mechanotransducing ion channels in astrocytes. Brain Res 584:272-86
Sigurdson, W; Ruknudin, A; Sachs, F (1992) Calcium imaging of mechanically induced fluxes in tissue-cultured chick heart: role of stretch-activated ion channels. Am J Physiol 262:H1110-5
Bowman, C L; Swann, J W; Severin, C M et al. (1992) Co-cultures of microglia and astrocytes from kainic acid-lesioned adult rat hippocampus: effects of glutamate. Glia 5:285-99
Sachs, F (1992) Stretch-sensitive ion channels: an update. Soc Gen Physiol Ser 47:241-60
Sachs, F (1991) Mechanical transduction by membrane ion channels: a mini review. Mol Cell Biochem 104:57-60
Sokabe, M; Sachs, F; Jing, Z Q (1991) Quantitative video microscopy of patch clamped membranes stress, strain, capacitance, and stretch channel activation. Biophys J 59:722-8
Izu, Y C; Sachs, F (1991) Inhibiting synthesis of extracellular matrix improves patch clamp seal formation. Pflugers Arch 419:218-20

Showing the most recent 10 out of 17 publications