Abstract

We are engaged in recording the properties of ionic channels that are activated by chemical means, particularly post synaptic receptors, in tissue cultured nerve and muscle. Using fluctuation analysis to estimate single channel conductance and channel open time for a variety of channels activated by different drugs, we will also measure voltage sensitivity if possible using fluctuation techniques and voltage clamp step procedures. We will look at how different pharmacological agents, particularly central nervous system active drugs, modify post synpatic channel function. We will also attempt to record the opening and closing of single channels in order to observe channel transitions in a theory free manner.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK037792-13
Application #
3236806
Study Section
Physiology Study Section (PHY)
Project Start
1977-09-01
Project End
1992-03-31
Budget Start
1990-04-01
Budget End
1992-03-31
Support Year
13
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
State University of New York at Buffalo
Department
Type
Schools of Medicine
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260

  Publications

Ruknudin, A; Sachs, F; Bustamante, J O (1993) Stretch-activated ion channels in tissue-cultured chick heart. Am J Physiol 264:H960-72
Ding, J P; Pickard, B G (1993) Mechanosensory calcium-selective cation channels in epidermal cells. Plant J 3:83-110
Ding, J P; Pickard, B G (1993) Mechanosensory calcium-selective cation channels in epidermal cells. Plant J 3:83-110
Bowman, C L; Ding, J P; Sachs, F et al. (1992) Mechanotransducing ion channels in astrocytes. Brain Res 584:272-86
Sigurdson, W; Ruknudin, A; Sachs, F (1992) Calcium imaging of mechanically induced fluxes in tissue-cultured chick heart: role of stretch-activated ion channels. Am J Physiol 262:H1110-5
Bowman, C L; Swann, J W; Severin, C M et al. (1992) Co-cultures of microglia and astrocytes from kainic acid-lesioned adult rat hippocampus: effects of glutamate. Glia 5:285-99
Sachs, F (1992) Stretch-sensitive ion channels: an update. Soc Gen Physiol Ser 47:241-60
Jing, Z Q; Sachs, F (1991) Alignment of tomographic projections using an incomplete set of fiducial markers. Ultramicroscopy 35:37-43
Bustamante, J O; Ruknudin, A; Sachs, F (1991) Stretch-activated channels in heart cells: relevance to cardiac hypertrophy. J Cardiovasc Pharmacol 17 Suppl 2:S110-3
Ruknudin, A; Song, M J; Sachs, F (1991) The ultrastructure of patch-clamped membranes: a study using high voltage electron microscopy. J Cell Biol 112:125-34

Showing the most recent 10 out of 17 publications