investigators's abstract) The overall objective of this proposal is to further the understanding of the determinants that regulate globin mRNA stability. This proposal has three main aims: 1) to identify all the alpha complex proteins both biochemically and genetically; 2) to functionally characterize the alpha-globin mRNA stability mechanisms by using an alpha-complex dependent in vitro mRNA decay system; and 3) to identify additional mRNA(s) bound by and possibly regulated by the alpha complex.
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Rodgers, Nancy D; Jiao, Xinfu; Kiledjian, Megerditch (2002) Identifying mRNAs bound by RNA-binding proteins using affinity purification and differential display. Methods 26:115-22 |
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