Abstract

investigators's abstract) The overall objective of this proposal is to further the understanding of the determinants that regulate globin mRNA stability. This proposal has three main aims: 1) to identify all the alpha complex proteins both biochemically and genetically; 2) to functionally characterize the alpha-globin mRNA stability mechanisms by using an alpha-complex dependent in vitro mRNA decay system; and 3) to identify additional mRNA(s) bound by and possibly regulated by the alpha complex.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK051611-04
Application #
6178042
Study Section
Hematology Subcommittee 2 (HEM)
Program Officer
Badman, David G
Project Start
1997-05-01
Project End
2002-04-30
Budget Start
2000-05-01
Budget End
2001-04-30
Support Year
4
Fiscal Year
2000
Total Cost
$148,200
Indirect Cost

  Institution

Name
Rutgers University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
038633251
City
New Brunswick
State
NJ
Country
United States
Zip Code
08901

  Publications

Liu, Hudan; Kiledjian, Megerditch (2007) An erythroid-enriched endoribonuclease (ErEN) involved in alpha-globin mRNA turnover. Protein Pept Lett 14:131-6
Carr-Schmid, Anne; Jiao, Xinfu; Kiledjian, Megerditch (2006) Identification of mRNA bound to RNA binding proteins by differential display. Methods Mol Biol 317:299-314
Liu, Shin-Wu; Jiao, Xinfu; Liu, Hudan et al. (2004) Functional analysis of mRNA scavenger decapping enzymes. RNA 10:1412-22
Khanna, Richie; Kiledjian, Megerditch (2004) Poly(A)-binding-protein-mediated regulation of hDcp2 decapping in vitro. EMBO J 23:1968-76
Gu, Meigang; Fabrega, Carme; Liu, Shin-Wu et al. (2004) Insights into the structure, mechanism, and regulation of scavenger mRNA decapping activity. Mol Cell 14:67-80
Steiger, Michelle; Carr-Schmid, Anne; Schwartz, David C et al. (2003) Analysis of recombinant yeast decapping enzyme. RNA 9:231-8
Rodgers, Nancy D; Jiao, Xinfu; Kiledjian, Megerditch (2002) Identifying mRNAs bound by RNA-binding proteins using affinity purification and differential display. Methods 26:115-22
Liu, Hudan; Rodgers, Nancy D; Jiao, Xinfu et al. (2002) The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases. EMBO J 21:4699-708
Wang, Zuoren; Jiao, Xinfu; Carr-Schmid, Anne et al. (2002) The hDcp2 protein is a mammalian mRNA decapping enzyme. Proc Natl Acad Sci U S A 99:12663-8
Rodgers, Nancy D; Wang, Zuoren; Kiledjian, Megerditch (2002) Regulated alpha-globin mRNA decay is a cytoplasmic event proceeding through 3'-to-5' exosome-dependent decapping. RNA 8:1526-37

Showing the most recent 10 out of 18 publications