Dr. Wessling-Resnick's overall aim is to understand the mechanism of iron transport across membranes. This is a very important problem both from the clinical and basic biology point of view. She has identified a protein called SFT, which is required for the Transferrin independent transport of Fe in Xenopus oocytes and in a preerythroid cell-line K562. SFT has some similarities with the ABC or ATP-Binding Cassette transporters. The three major aims of this proposal are: (1) to characterize the role of SFT in Fe transport; (2) to understand how SFT is localized to both the plasma membrane and endosomes; and (3) to identify the cis-acting elements that regulate the intracellular levels of SFT.
|Simpson, Jeremy C; Griffiths, Gareth; Wessling-Resnick, Marianne et al. (2004) A role for the small GTPase Rab21 in the early endocytic pathway. J Cell Sci 117:6297-311|
|Akompong, T; Ramm, E; Chang, C et al. (1999) Immunological analysis of beta-thalassemic mouse intestinal proteins reveals up-regulation of sucrase-isomaltase in response to iron overload. J Nutr 129:949-52|
|Yu, J; Wessling-Resnick, M (1998) Influence of copper depletion on iron uptake mediated by SFT, a stimulator of Fe transport. J Biol Chem 273:6909-15|
|Yu, J; Yu, Z K; Wessling-Resnick, M (1998) Expression of SFT (stimulator of Fe transport) is enhanced by iron chelation in HeLa cells and by hemochromatosis in liver. J Biol Chem 273:34675-8|
|Gutierrez, J A; Inman, R S; Akompong, T et al. (1998) Metabolic depletion inhibits the uptake of nontransferrin-bound iron by K562 cells. J Cell Physiol 177:585-92|
|Yu, J; Wessling-Resnick, M (1998) Structural and functional analysis of SFT, a stimulator of Fe Transport. J Biol Chem 273:21380-5|
|Gutierrez, J A; Yu, J; Rivera, S et al. (1997) Functional expression cloning and characterization of SFT, a stimulator of Fe transport. J Cell Biol 139:895-905|