Dr. Wessling-Resnick's overall aim is to understand the mechanism of iron transport across membranes. This is a very important problem both from the clinical and basic biology point of view. She has identified a protein called SFT, which is required for the Transferrin independent transport of Fe in Xenopus oocytes and in a preerythroid cell-line K562. SFT has some similarities with the ABC or ATP-Binding Cassette transporters. The three major aims of this proposal are: (1) to characterize the role of SFT in Fe transport; (2) to understand how SFT is localized to both the plasma membrane and endosomes; and (3) to identify the cis-acting elements that regulate the intracellular levels of SFT.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK052371-01
Application #
2017886
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Haft, Carol Renfrew
Project Start
1997-05-15
Project End
2001-03-31
Budget Start
1997-05-15
Budget End
1998-03-31
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Harvard University
Department
Nutrition
Type
Schools of Public Health
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
Simpson, Jeremy C; Griffiths, Gareth; Wessling-Resnick, Marianne et al. (2004) A role for the small GTPase Rab21 in the early endocytic pathway. J Cell Sci 117:6297-311
Akompong, T; Ramm, E; Chang, C et al. (1999) Immunological analysis of beta-thalassemic mouse intestinal proteins reveals up-regulation of sucrase-isomaltase in response to iron overload. J Nutr 129:949-52
Yu, J; Wessling-Resnick, M (1998) Influence of copper depletion on iron uptake mediated by SFT, a stimulator of Fe transport. J Biol Chem 273:6909-15
Yu, J; Yu, Z K; Wessling-Resnick, M (1998) Expression of SFT (stimulator of Fe transport) is enhanced by iron chelation in HeLa cells and by hemochromatosis in liver. J Biol Chem 273:34675-8
Gutierrez, J A; Inman, R S; Akompong, T et al. (1998) Metabolic depletion inhibits the uptake of nontransferrin-bound iron by K562 cells. J Cell Physiol 177:585-92
Yu, J; Wessling-Resnick, M (1998) Structural and functional analysis of SFT, a stimulator of Fe Transport. J Biol Chem 273:21380-5
Gutierrez, J A; Yu, J; Rivera, S et al. (1997) Functional expression cloning and characterization of SFT, a stimulator of Fe transport. J Cell Biol 139:895-905