Diamond-based electrodes are a promising technology for biomedical research. We propose to develop conductive, diamond-based materials as superior electrodes for extracellular, neurological sensing and stimulation. Diamond provides a unique opportunity to integrate stimulation and sensing in the same device. We hypothesize that diamond electrodes will provide real-time neurological sensing capability, with greatly improved sensitivity, selectivity and stability, as well as an expanded potential range of operation over present materials. Diamond may expand neural stimulation capabilities by avoiding side reactions that lead to tissue damage and by providing long-term stability. These advantages will be applicable to a broad variety of neurological systems. These capabilities will be explored through three specific aims focusing on extracellular sensing of dopamine, adenosine, and serotonin and evaluation of neural stimulation, using our diamond technology toward addressing specific neuromodulatory questions. First, we will characterize the electrochemical behavior of a diamond microelectrode with chemically modified surfaces for neurological sensing and neural stimulation capabilities. Second, we will use our characterized diamond electrode to separately measure dopamine, serotonin, and adenosine concentration changes during in vitro neural activity. Each of these applications is opportunity for diamond electrodes to make an immediate impact on understanding neuromodulation in these specific neural circuits. Third, two diamond electrodes will be used to simultaneously stimulate neural activity of single cells and monitor serotonin release in the Aplysia californica. Relevance to Public Health: Diamond is a new tool to aid in our understanding of neurotransmission, and could be incorporated into advanced neuroprosthetic or drug therapy devices. This proposed project should provide the basis of a whole class of robust, implantable diamond-based devices for neurological applications, which could be extended into broader areas of biomedical research. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB004018-02
Application #
7257247
Study Section
Special Emphasis Panel (ZRG1-MDCN-K (50))
Program Officer
Henderson, Lori
Project Start
2006-07-04
Project End
2010-04-30
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
2
Fiscal Year
2007
Total Cost
$332,781
Indirect Cost
Name
Case Western Reserve University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Hudak, Eric M; Kumsa, Doe W; Martin, Heidi B et al. (2017) Electron transfer processes occurring on platinum neural stimulating electrodes: calculated charge-storage capacities are inaccessible during applied stimulation. J Neural Eng 14:046012
Halpern, Jeffrey M; Martin, Heidi B (2014) Rhenium Alloys as Ductile Substrates for Diamond Thin-Film Electrodes. Diam Relat Mater 42:33-40
Azizi, Farouk; Lu, Hui; Chiel, Hillel J et al. (2010) Chemical neurostimulation using pulse code modulation (PCM) microfluidic chips. J Neurosci Methods 192:193-8
Hudak, E M; Mortimer, J T; Martin, H B (2010) Platinum for neural stimulation: voltammetry considerations. J Neural Eng 7:26005
Roham, Masoud; Halpern, Jeffrey M; Martin, Heidi B et al. (2008) Wireless amperometric neurochemical monitoring using an integrated telemetry circuit. IEEE Trans Biomed Eng 55:2628-34
Lu, Hui; Chestek, Cynthia A; Shaw, Kendrick M et al. (2008) Selective extracellular stimulation of individual neurons in ganglia. J Neural Eng 5:287-309
Roham, Masoud; Halpern, Jeffrey M; Martin, Heidi B et al. (2007) Diamond microelectrodes and CMOS microelectronics for wireless transmission of fast-scan cyclic voltammetry. Conf Proc IEEE Eng Med Biol Soc 2007:6044-7