The goal of this research program is to develop a mass spectrometry technique for the determination of bioaerosol concentration in air. The work is divided into three parts. First, an off-line method for microbe and bioaerosol ionization will be developed which uses a liquid matrix for matrix-assisted laser desorption ionization (MALDI). Second, the liquid matrix method will be used with an on-line aerosol mass spectrometer that detects single particles in a known air volume and tallies the number of bioaerosol particles. Third, bioaerosols will be collected and cultured to determine the number of colony forming units (CFU) in a given volume. The results will be compared to those obtained with the aerosol mass spectrometer.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
7R01ES010497-02
Application #
6548937
Study Section
Special Emphasis Panel (ZRG1-BMT (01))
Program Officer
Gray, Kimberly A
Project Start
2001-01-08
Project End
2003-12-31
Budget Start
2001-08-01
Budget End
2001-12-31
Support Year
2
Fiscal Year
2001
Total Cost
$153,023
Indirect Cost
Name
Louisiana State University A&M Col Baton Rouge
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803
Jackson, Shelley N; Kim, Jae-Kuk; Laboy, Jorge L et al. (2006) Particle formation by infrared laser ablation of glycerol: implications for ion formation. Rapid Commun Mass Spectrom 20:1299-304
Kim, Jae-Kuk; Jackson, Shelley N; Murray, Kermit K (2005) Matrix-assisted laser desorption/ionization mass spectrometry of collected bioaerosol particles. Rapid Commun Mass Spectrom 19:1725-9
Jackson, Shelley N; Mishra, Sushama; Murray, Kermit K (2004) On-line laser desorption/ionization mass spectrometry of matrix-coated aerosols. Rapid Commun Mass Spectrom 18:2041-5
Jackson, Shelley N; Dutta, Sucharita M; Murray, Kermit K (2004) A nitrocellulose matrix for infrared matrix-assisted laser desorption/ionization of polycyclic aromatic hydrocarbons. Rapid Commun Mass Spectrom 18:228-30
Jackson, Shelley N; Murray, Kermit K (2002) Matrix addition by condensation for matrix-assisted laser desorption/ionization of collected aerosol particles. Anal Chem 74:4841-4