The goal of this research program is to develop a mass spectrometry technique for the determination of bioaerosol concentration in air. The work is divided into three parts. First, an off-line method for microbe and bioaerosol ionization will be developed which uses a liquid matrix for matrix-assisted laser desorption ionization (MALDI). Second, the liquid matrix method will be used with an on-line aerosol mass spectrometer that detects single particles in a known air volume and tallies the number of bioaerosol particles. Third, bioaerosols will be collected and cultured to determine the number of colony forming units (CFU) in a given volume. The results will be compared to those obtained with the aerosol mass spectrometer.
| Jackson, Shelley N; Kim, Jae-Kuk; Laboy, Jorge L et al. (2006) Particle formation by infrared laser ablation of glycerol: implications for ion formation. Rapid Commun Mass Spectrom 20:1299-304 |
| Kim, Jae-Kuk; Jackson, Shelley N; Murray, Kermit K (2005) Matrix-assisted laser desorption/ionization mass spectrometry of collected bioaerosol particles. Rapid Commun Mass Spectrom 19:1725-9 |
| Jackson, Shelley N; Mishra, Sushama; Murray, Kermit K (2004) On-line laser desorption/ionization mass spectrometry of matrix-coated aerosols. Rapid Commun Mass Spectrom 18:2041-5 |
| Jackson, Shelley N; Dutta, Sucharita M; Murray, Kermit K (2004) A nitrocellulose matrix for infrared matrix-assisted laser desorption/ionization of polycyclic aromatic hydrocarbons. Rapid Commun Mass Spectrom 18:228-30 |
| Jackson, Shelley N; Murray, Kermit K (2002) Matrix addition by condensation for matrix-assisted laser desorption/ionization of collected aerosol particles. Anal Chem 74:4841-4 |
