Abstract

The intraocular kinetics of a range of fluorescent dyes in the rabbit eye will be established and related to their physicochemical properties. It is intended that this will aid in the design of therapeutic drugs having desired kinetic properties in the eye. The kinetics will be analyzed in terms of the permeabilities of the intraocular cellular barriers and diffusion in and binding by the tissues. Particular attention will be paid to the influence of the crystalline lens on kinetics. The dyes to be investigated will include fluorones, rhodamines, oxazines, carbocyanines, merocyanines and pyrenes. Their molecular weight, partition coefficient, ionic charge and pK will be established, and correlations between these properties and their permeabilities, etc. in the eye will be looked for.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004863-03
Application #
3259420
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1983-07-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Kumar, J P (2009) The sine oculis homeobox (SIX) family of transcription factors as regulators of development and disease. Cell Mol Life Sci 66:565-83
Srinivas, S P (1994) In situ measurement of fluorescein release by collagen shields in human eyes. Curr Eye Res 13:281-8
Maurice, D M; Srinivas, S P (1992) Use of fluorometry in assessing the efficacy of a cation-sensitive gel as an ophthalmic vehicle: comparison with scintigraphy. J Pharm Sci 81:615-9
Macdonald, E A; Maurice, D M (1991) The kinetics of tear fluid under the lower lid. Exp Eye Res 53:421-5
Araie, M; Maurice, D M (1991) The loss of fluorescein, fluorescein glucuronide and fluorescein isothiocyanate dextran from the vitreous by the anterior and retinal pathways. Exp Eye Res 52:27-39
Macdonald, E A; Maurice, D M (1991) Loss of fluorescein across the conjunctiva. Exp Eye Res 53:427-30
Eliason, J A; Maurice, D M (1990) Staining of the conjunctiva and conjunctival tear film. Br J Ophthalmol 74:519-22
Chen, F S; Maurice, D M (1990) The pH in the precorneal tear film and under a contact lens measured with a fluorescent probe. Exp Eye Res 50:251-9
Araie, M; Maurice, D (1987) The rate of diffusion of fluorophores through the corneal epithelium and stroma. Exp Eye Res 44:73-87
Maurice, D M (1987) Flow of water between aqueous and vitreous compartments in the rabbit eye. Am J Physiol 252:F104-8

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