The intraocular kinetics of a range of fluorophores after topical application to the rabbit eye will be investigated by measuring the changes with time in concentration in the cornea and aqueous with a specially designed slit-lamp fluorometer. Similar measurements will be made on the isolated cornea, where dynamic measurements of fluorescent profiles in the cornea will be made with a modified specular microscope. The following biological parameters for the dyes will be determined: Epithelial permeability, solubility, transit time, endothelial permeability, stromal solubility, diffusion rate. The oil-water partition coefficient, ionic charge and molecular weights of the dyes will be correlated with their biological properties in an attempt to establish relationships that will assist in the development of therapeutic drugs with improved pharmacokinetic properties. Measurements of the kinetics of the fluorophores in the crystalline lens will be included in the measurements on the intact rabbit in order to determine to what extent it can serve as a reservoir for drugs which will even out the troughs in concentration in the anterior chamber that occur between applications. The changes in the natural fluorescence in the human cornea with age, as well as in disease, will be followed with the slit-lamp fluorophotometer using different excitation wavelengths. It is hoped to identify a quantifiable marker for age or the diagnosis of pathological conditions. The influence of the age of the host on the aging of a corneal graft will be examined.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY004863-06
Application #
3259422
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1983-07-01
Project End
1989-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
6
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
Kumar, J P (2009) The sine oculis homeobox (SIX) family of transcription factors as regulators of development and disease. Cell Mol Life Sci 66:565-83
Srinivas, S P (1994) In situ measurement of fluorescein release by collagen shields in human eyes. Curr Eye Res 13:281-8
Maurice, D M; Srinivas, S P (1992) Use of fluorometry in assessing the efficacy of a cation-sensitive gel as an ophthalmic vehicle: comparison with scintigraphy. J Pharm Sci 81:615-9
Macdonald, E A; Maurice, D M (1991) The kinetics of tear fluid under the lower lid. Exp Eye Res 53:421-5
Araie, M; Maurice, D M (1991) The loss of fluorescein, fluorescein glucuronide and fluorescein isothiocyanate dextran from the vitreous by the anterior and retinal pathways. Exp Eye Res 52:27-39
Macdonald, E A; Maurice, D M (1991) Loss of fluorescein across the conjunctiva. Exp Eye Res 53:427-30
Eliason, J A; Maurice, D M (1990) Staining of the conjunctiva and conjunctival tear film. Br J Ophthalmol 74:519-22
Chen, F S; Maurice, D M (1990) The pH in the precorneal tear film and under a contact lens measured with a fluorescent probe. Exp Eye Res 50:251-9
Araie, M; Maurice, D (1987) The rate of diffusion of fluorophores through the corneal epithelium and stroma. Exp Eye Res 44:73-87
Maurice, D M (1987) Flow of water between aqueous and vitreous compartments in the rabbit eye. Am J Physiol 252:F104-8

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