Abstract

The location of retinyl palmitate hydrolase in the eye will be determined by immunohistochemical techniques. The retinyl palmitate hydrolase will first be purified to homogeneity from bovine tissue. Rabbit will be used to produce antibody against this enzyme. Sections of the bovine eye will be incubated with antiserum specific to the retinyl palmitate hydrolase. The location of the antigen-antibody complex will be visualized by specific staining and reveals whether this enzyme is found in the photoreceptors, Muller cells, other cells of the retina and/or in the cells of the retinal pigment epithelium. The results of this project will provide information important to our current understanding on the origin of retinoids to form visual pigments in the eye.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY006438-02
Application #
3262525
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1986-09-30
Project End
1989-09-29
Budget Start
1987-09-30
Budget End
1988-09-29
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
Schools of Arts and Sciences
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78249

  Publications

Mata, N L; Tsin, A T; Chambers, J P (1992) Hydrolysis of 11-cis- and all-trans-retinyl palmitate by retinal pigment epithelium microsomes. J Biol Chem 267:9794-9
Tsin, A T; Malsbury, D W (1992) Bile salt independent retinyl ester hydrolases in the bovine eye. Brain Res Bull 28:121-5
Sanders-Sanchez, S R; Malsbury, D; Tsin, A T (1990) Comparison of retinal pigment epithelium cell preparations from the bovine eye. Experientia 46:498-500
Chambers, J P; Kumar, P; Tsin, A T et al. (1990) Partial characterization of a high affinity [Ca2+ + Mg2+]-dependent adenosinetriphosphatase from bovine retina. Exp Eye Res 50:127-34
Tsin, A T; Gentles, S N; Castillo, E A (1989) Selective utilization of serum vitamin A for visual pigment synthesis. J Exp Biol 142:207-14
Phillips, T S; Tsin, A T; Reiter, R J et al. (1989) Retinoids in the bovine pineal gland. Brain Res Bull 22:259-61
Rodriguez, K A; Tsin, A T (1989) Retinyl esters in the vertebrate neuroretina. Am J Physiol 256:R255-8
Tsin, A T; Chambers, J P (1988) Light and temperature affect retinyl ester hydrolase activity and visual pigment composition. Experientia 44:20-1
Tsin, A T; Pedrozo-Fernandez, H A; Gallas, J M et al. (1988) The fluorescence quantum yield of vitamin A2. Life Sci 43:1379-84
Tsin, A T; Flores, J M; Rodriguez, K A (1987) Visual pigments and retinoids in the Mongolian jird. Life Sci 41:2085-90