Vesicular membranes are intimately involved in the storage, transport and release of regulatory and metabolic agents in cellular systems. To function properly these vesicles must be able to actively accumulate these agents, retain them for periods of time and then expel them to an external environment by fusion with a limiting membrane. It is our objective to understand at a molecular level factors which affect the fusion, solute concentration, and solute transport properties of vesicles and to use that understanding in the design of useful artificial analogues. These objectives will be pursued largely through the study of model phospholipid vesicles which contain anionic lipids and in which fusion can be stimulated by the addition of Ca2 ion. However, the studies will also be extended to isolated storage granules in which biogenic amines are concentrated. Nuclear magnetic resonance (nmr) techniques including 1H, 19F, 31P, 13C, and 113Cd nmr will be developed and used in the study of both membrane structure and membrane transport.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM019035-14
Application #
3269472
Study Section
(SSS)
Project Start
1978-01-01
Project End
1986-08-31
Budget Start
1985-01-01
Budget End
1986-08-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Yale University
Department
Type
Schools of Arts and Sciences
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code