Abstract

Research will be undertaken to employ ion and gas sensing membrane electrodes for clinical analysis of substrates, enzymes, electrolytes, and other body fluid constituents. Methods will be developed for the determination of clinically important substances in serum, whole blood, and urine involving the employment of crystal membrane, enzyme coated and other electrodes in continuous analysis systems with capabilities of handling 100-200 samples per hour. It is expected that the proposed approach will result in desirable simplifications of the chemistries involved, by elimination of dialysis and color development steps, and will produce a significant reduction in analysis costs, by the elimination of expensive optical and electronic instrumentation as well as a reduction in the quantities and number of required reagents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM025308-08
Application #
3272924
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1978-01-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
8
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Delaware
Department
Type
Schools of Arts and Sciences
DUNS #
059007500
City
Newark
State
DE
Country
United States
Zip Code
19716

  Publications

Smit, M H; Rechnitz, G A (1993) Toxin detection using a tyrosinase-coupled oxygen electrode. Anal Chem 65:380-5
Chen, L; Martin, G B; Rechnitz, G A (1992) Microtiter plate binding assay for cholinergic compounds utilizing the nicotinic acetylcholine receptor. Anal Chem 64:3018-23
Takeuchi, T; Tanaka, S; Rechnitz, G A (1992) Biotinylated 1012-S conjugate as a probe ligand for benzodiazepine receptors: characterization of receptor binding sites and receptor assay for benzodiazepine drugs. Anal Biochem 203:158-62
Tanaka, S; Takeuchi, T; Rechnitz, G A (1992) Non-isotopic receptor assay for benzodiazepines using a biotin-labeled ligand and biotin-immobilized microtiter plate. J Chromatogr 597:443-8
Smit, M H; Rechnitz, G A (1992) Reagentless enzyme electrode for the determination of manganese through biocatalytic enhancement. Anal Chem 64:245-9
Takeuchi, T; Rechnitz, G A (1991) Nonisotopic receptor-binding assay for benzodiazepine receptors utilizing a fluorophore labeled ligand. Anal Biochem 194:250-5
Takeuchi, T; Rechnitz, G A (1990) Enzymatic solid-phase assay for biotin and a biotin-benzodiazepine conjugate. Bioconjug Chem 1:227-30
Hallowell, S F; Rechnitz, G A (1990) Enzyme-amplified receptor assay screening test for chlorpromazine, trifluoperazine, and phencyclidine. J Clin Lab Anal 4:64-73
Ho, M Y; Rechnitz, G A (1987) Highly stable biosensor using an artificial enzyme. Anal Chem 59:536-7
Yao, T; Rechnitz, G A (1987) Potentiometric biosensor for riboflavin based on the use of aporiboflavin-binding protein. Anal Chem 59:2115-8

Showing the most recent 10 out of 12 publications