Abstract

Chorion (eggshell) protein genes isolated from Drosophila melanogaster will be mutated in vitro and reintroduced into the germ line by P element-mediated transformation. Strains containing single transferred genes at defined chromosomal sites will be isolated and the expression of the newly integrated gene in developing ovarian follicle cells studied. Specific changes in gene structure will be related to the ability of the chorion gene to undergo tissue and developmental stage specific amplification and transcription. The effect of the local chromosomal environment in diverse genomic regions on these properties will also be examined. Knowledge of the mechanisms which regulate and program gene replication and transcription during development is essential to understanding the etiology of birth defects, aging and acquired disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM027875-07
Application #
3275096
Study Section
Genetics Study Section (GEN)
Project Start
1980-04-01
Project End
1988-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Carnegie Institution of Washington, D.C.
Department
Type
DUNS #
072641707
City
Washington
State
DC
Country
United States
Zip Code
20005

  Publications

Kotova, Elena; Jarnik, Michael; Tulin, Alexei V (2010) Uncoupling of the transactivation and transrepression functions of PARP1 protein. Proc Natl Acad Sci U S A 107:6406-11
Ji, Yingbiao; Tulin, Alexei V (2009) Poly(ADP-ribosyl)ation of heterogeneous nuclear ribonucleoproteins modulates splicing. Nucleic Acids Res 37:3501-13
Kotova, Elena; Jarnik, Michael; Tulin, Alexei V (2009) Poly (ADP-ribose) polymerase 1 is required for protein localization to Cajal body. PLoS Genet 5:e1000387
Pinnola, Aaron; Naumova, Natasha; Shah, Meera et al. (2007) Nucleosomal core histones mediate dynamic regulation of poly(ADP-ribose) polymerase 1 protein binding to chromatin and induction of its enzymatic activity. J Biol Chem 282:32511-9
Tulin, Alexei; Chinenov, Yurii; Spradling, Allan (2003) Regulation of chromatin structure and gene activity by poly(ADP-ribose) polymerases. Curr Top Dev Biol 56:55-83
Tulin, Alexei; Spradling, Allan (2003) Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci. Science 299:560-2
Tulin, Alexei; Stewart, Dianne; Spradling, Allan C (2002) The Drosophila heterochromatic gene encoding poly(ADP-ribose) polymerase (PARP) is required to modulate chromatin structure during development. Genes Dev 16:2108-19
Calvi, B R; Spradling, A C (2001) The nuclear location and chromatin organization of active chorion amplification origins. Chromosoma 110:159-72
Lilly, M A; de Cuevas, M; Spradling, A C (2000) Cyclin A associates with the fusome during germline cyst formation in the Drosophila ovary. Dev Biol 218:53-63
Dej, K J; Spradling, A C (1999) The endocycle controls nurse cell polytene chromosome structure during Drosophila oogenesis. Development 126:293-303

Showing the most recent 10 out of 36 publications