The objective of this proposal is to develop techniques for the analysis of the DNA structure of human chromosome 11. The experimental approach involves the comparison of maps of the chromosome derived by three different approaches. 1. Genetic linkage among sites on the chromosome defined by restriction fragment length polymorphisms. 2. Physical association of specific DNA segments in somatic cell hybrids carrying limited regions of the chromosome derived either by selection of hybrids carrying the entire chromosome or by metaphase chromosome transfer. 3. Karyotypic analysis and in situ hybridization of cloned DNA segments to extend metaphase chromosomes. The immediate objectives of the proposal are: 1. To compare genetic map distance to physical distance in nucleotides in a specific region of the chromosome. 2. To isolate DNA segments which cover a contiguous region of several million base pairs of the chromosome. 3. To further develop methods utilizing interspersed repeated DNA sequences as probes to map specific regions of the chromosome. 4. To further develop isolated metaphase chromosome transfer as a tool for mapping regions of the chromosome.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM027882-07
Application #
3275104
Study Section
Molecular Biology Study Section (MBY)
Project Start
1980-04-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
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