Abstract

The objective of this proposal is to develop techniques for the analysis of the DNA structure of human chromosome 11. The experimental approach involves the comparison of maps of the chromosome derived by three different approaches. 1. Genetic linkage among sites on the chromosome defined by restriction fragment length polymorphisms. 2. Physical association of specific DNA segments in somatic cell hybrids carrying limited regions of the chromosome derived either by selection of hybrids carrying the entire chromosome or by metaphase chromosome transfer. 3. Karyotypic analysis and in situ hybridization of cloned DNA segments to extend metaphase chromosomes. The immediate objectives of the proposal are: 1. To compare genetic map distance to physical distance in nucleotides in a specific region of the chromosome. 2. To isolate DNA segments which cover a contiguous region of several million base pairs of the chromosome. 3. To further develop methods utilizing interspersed repeated DNA sequences as probes to map specific regions of the chromosome. 4. To further develop isolated metaphase chromosome transfer as a tool for mapping regions of the chromosome.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM027882-08
Application #
3275105
Study Section
Molecular Biology Study Section (MBY)
Project Start
1980-04-01
Project End
1988-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
8
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Park, S; Schalling, M; Bernard, A et al. (1993) The Wilms tumour gene WT1 is expressed in murine mesoderm-derived tissues and mutated in a human mesothelioma. Nat Genet 4:415-20
Oettinger, M A; Stanger, B; Schatz, D G et al. (1992) The recombination activating genes, RAG 1 and RAG 2, are on chromosome 11p in humans and chromosome 2p in mice. Immunogenetics 35:97-101
Haber, D A; Timmers, H T; Pelletier, J et al. (1992) A dominant mutation in the Wilms tumor gene WT1 cooperates with the viral oncogene E1A in transformation of primary kidney cells. Proc Natl Acad Sci U S A 89:6010-4
Schwartz, C E; Haber, D A; Stanton, V P et al. (1991) Familial predisposition to Wilms tumor does not segregate with the WT1 gene. Genomics 10:927-30
Pelletier, J; Schalling, M; Buckler, A J et al. (1991) Expression of the Wilms' tumor gene WT1 in the murine urogenital system. Genes Dev 5:1345-56
Warburton, D; Gersen, S; Yu, M T et al. (1990) Monochromosomal rodent-human hybrids from microcell fusion of human lymphoblastoid cells containing an inserted dominant selectable marker. Genomics 6:358-66
Glaser, T; Rose, E; Morse, H et al. (1990) A panel of irradiation-reduced hybrids selectively retaining human chromosome 11p13: their structure and use to purify the WAGR gene complex. Genomics 6:48-64
Glaser, T; Lane, J; Housman, D (1990) A mouse model of the aniridia-Wilms tumor deletion syndrome. Science 250:823-7
Lichter, P; Tang, C J; Call, K et al. (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64-9
Glaser, T; Housman, D; Lewis, W H et al. (1989) A fine-structure deletion map of human chromosome 11p: analysis of J1 series hybrids. Somat Cell Mol Genet 15:477-501

Showing the most recent 10 out of 22 publications