The comparative structure and organization of homologous gene regions from two species of non-human primates, the baboon and gorilla, will be studied in an effort to define sequences associated with globin gene regulation. Recombinant DNA """"""""libraries"""""""" of genomic DNA for each species will be constructed and screened with human globin cDNA probes, and phage containing alpha, beta, delta, gamma, and epsilon gene sequences will be detected and cloned. Both the alpha and non-alpha gene clusters from these primates will be compared to those of humans with respect to the structural organization of both expressed and non-expressed DNA regions, particularly the putative regulatory areas inferred from studies of human thalassemias. The active delta gene of the gorilla, the silent delta gene of the baboon, and the occasionally active 3-alpha gene of the gorilla will each be studied by nucleotide sequence analysis. Detailed restriction mapping of the twin alpha and twin gamma loci in each species will be performed to determine whether they evolve in """"""""concert"""""""" by unequal crossing-over and loss. The complexity and organization of repeated DNA families within the alpha and non-alpha gene clusters will be compared between species and with man to determine the evolutionary stability of such sequences and whether they can be correlated with gene function.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM028931-08
Application #
3276310
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1981-04-01
Project End
1990-11-30
Budget Start
1988-12-01
Budget End
1990-11-30
Support Year
8
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Scott, A F; Elizaga, A; Morrell, J et al. (1994) Characterization of a gene coamplified with Ki-ras in Y1 murine adrenal carcinoma cells that codes for a putative membrane protein. Genomics 20:227-30
Mathias, S L; Scott, A F (1993) Promoter binding proteins of an active human L1 retrotransposon. Biochem Biophys Res Commun 191:625-32
Mathias, S L; Scott, A F; Kazazian Jr, H H et al. (1991) Reverse transcriptase encoded by a human transposable element. Science 254:1808-10
Fridman, R; Scott, A F; Muller, D et al. (1990) The role of cell adhesion and migration in the in vitro invasiveness of mouse adrenal carcinoma cells. Invasion Metastasis 10:208-24
Penno, M B; Passaniti, A; Fridman, R et al. (1989) In vitro galactosylation of a 110-kDa glycoprotein by an endogenous cell surface galactosyltransferase correlates with the invasiveness of adrenal carcinoma cells. Proc Natl Acad Sci U S A 86:6057-61
Scott, A F; Schmeckpeper, B J; Abdelrazik, M et al. (1987) Origin of the human L1 elements: proposed progenitor genes deduced from a consensus DNA sequence. Genomics 1:113-25
George, D L; Scott, A F; Trusko, S et al. (1985) Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells. EMBO J 4:1199-203