Abstract

One of the major questions being pursued in developmental biology today is, """"""""How are specific genes turned on and off at the appropriated times in a developmental sequence?"""""""" The ultimate goal of the research contained in this proposal is to study the processes of differentiation in the life cycle of the dimorphic bacterium Caulobacter crescentus and to attempt to understand the modes of regulation controlling these processes. Work carried out in our laboratory so far has resulted in the development of techniques for the genetic analysis of C. crescentus, the isolation of mutants, the construction of a genetic map and the construction of a clone bank of C. crescentus genes. These results are being used in our laboratory and elsewhere to study specific C. crescentus genes, but more work is needed to maximize the potential for the use of genetic tools to study the temperal control of gene regulation in C. crescentus. Therefore, we propose: 1) To refine and improve the C. crescentus genetic map: a) by precise mapping of areas of uncertainty and; b) by mapping of additional genes. 2) To develop new vectors for transposon mutagenesis. 3) To establish the circularity of the C. crescentus chromosome. 4) To perform fine structure analysis of specific regions of the C. crescentus chromosome.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM033580-03
Application #
3283485
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1983-09-23
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of South Carolina at Columbia
Department
Type
Schools of Arts and Sciences
DUNS #
111310249
City
Columbia
State
SC
Country
United States
Zip Code
29208

  Publications

Wang, S P; Sharma, P L; Schoenlein, P V et al. (1993) A histidine protein kinase is involved in polar organelle development in Caulobacter crescentus. Proc Natl Acad Sci U S A 90:630-4
Schoenlein, P V; Lui, J; Gallman, L et al. (1992) The Caulobacter crescentus flaFG region regulates synthesis and assembly of flagellin proteins encoded by two genetically unlinked gene clusters. J Bacteriol 174:6046-53
Khambaty, F M; Ely, B (1992) Molecular genetics of the flgI region and its role in flagellum biosynthesis in Caulobacter crescentus. J Bacteriol 174:4101-9
Driks, A; Schoenlein, P V; DeRosier, D J et al. (1990) A Caulobacter gene involved in polar morphogenesis. J Bacteriol 172:2113-23
Ely, B; Ely, T W; Gerardot, C J et al. (1990) Circularity of the Caulobacter crescentus chromosome determined by pulsed-field gel electrophoresis. J Bacteriol 172:1262-6
Schoenlein, P V; Gallman, L S; Ely, B (1989) Organization of the flaFG gene cluster and identification of two additional genes involved in flagellum biogenesis in Caulobacter crescentus. J Bacteriol 171:1544-53
Schoenlein, P V; Ely, B (1989) Characterization of strains containing mutations in the contiguous flaF, flbT, or flbA-flaG transcription unit and identification of a novel fla phenotype in Caulobacter crescentus. J Bacteriol 171:1554-61
Ely, B; Ely, T W (1989) Use of pulsed field gel electrophoresis and transposon mutagenesis to estimate the minimal number of genes required for motility in Caulobacter crescentus. Genetics 123:649-54
Ely, B; Gerardot, C J (1988) Use of pulsed-field-gradient gel electrophoresis to construct a physical map of the Caulobacter crescentus genome. Gene 68:323-33
Bryan, R; Champer, R; Gomes, S et al. (1987) Separation of temporal control and trans-acting modulation of flagellin and chemotaxis genes in Caulobacter. Mol Gen Genet 206:300-6

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