The objective of this research is an understanding of the lipid-protein interactions through the study of three protein systems: phosopholipase A2, melittin, and a sterol carrier protein. Studies on phospholipase A2 include the isolation of a membrane-bound enzyme from sheep erythrocytes and its characterization in lipid monolayer assay systems. Studies with melittin involve mainly interactions of the peptide with lipid monolayer systems. Studies of interactions of a sterol carrier protein with micellar and liposomal lipid systems involve fluorescence and gel filtration techniques.
| Hendrickson, H S (1991) Phospholipase A2 assays with fluorophore-labeled lipid substrates. Methods Enzymol 197:90-4 |
| Hendrickson, H S; Hendrickson, E K (1990) A facile asymmetric synthesis of glycerol phospholipids via tritylglycidol prepared by the asymmetric epoxidation of allyl alcohol. Thiolester and thioether analogs of phosphatidylcholine. Chem Phys Lipids 53:115-20 |
| Hendrickson, H S; Kotz, K J; Hendrickson, E K (1990) Evaluation of fluorescent and colored phosphatidylcholine analogs as substrates for the assay of phospholipase A2. Anal Biochem 185:80-3 |
| Hendrickson, H S; Hendrickson, E K; Rustad, T J (1987) Synthesis of a naphthylvinyl-labeled glycerol ether analog of phosphatidylcholine and its use in the assay of phospholipase A2. J Lipid Res 28:864-72 |
| Hendrickson, H S; Dumdei, E J; Batchelder, A G et al. (1987) Synthesis of prodan-phosphatidylcholine, a new fluorescent probe, and its interactions with pancreatic and snake venom phospholipases A2. Biochemistry 26:3697-703 |
