Abstract

A series of enzymological, stereochemical and mechanistic studies are proposed on reactions in hexose metabolism, which lead to the reductive replacement of hydroxyl groups by hydrogen and to the epimerization of chiral centers in the carbohydrate chain. These studies will deal with the GDP-mannose oxidoreductase reaction involved in fucose biosynthesis, with the CDP-4-keto-6-deoxyglucose 3-reductase system from Salmonella, with the formation of 2,6-dideoxyhexoses in several streptomyces, with the epimerization of GDP-mannose to GDP-L-galactose (and GDP-L-fucose) by plant enzymes, and with the epimerization of D mannuronic to L guluronic acid residues in the course of alginic acid formation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034387-03
Application #
3285276
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1985-09-17
Project End
1989-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Ohio State University
Department
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210

  Publications

Steginsky, C A; Beale, J M; Floss, H G et al. (1992) Structural determination of alginic acid and the effects of calcium binding as determined by high-field n.m.r. Carbohydr Res 225:11-26
Thompson, M W; Strohl, W R; Floss, H G (1992) Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes. J Gen Microbiol 138:779-86
Oths, P J; Mayer, R M; Floss, H G (1990) Stereochemistry and mechanism of the GDP-mannose dehydratase reaction. Carbohydr Res 198:91-100