Abstract

The program is concerned with the detection and analysis of sequence variations in the human genome, particularly those responsible for genetic disease or useful in following those variants. We will depend largely on the system of sequence-defined DNA separations of Fischer and Lerman and extend the system, both in its experimental capability and in theoretical basis of its function. We will also pursue certain structural and thermodynamic questions, particularly those bearing on the relations between sequence and the stability of the helix with respect to melting.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM035095-02
Application #
3287197
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1985-09-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
2
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Genetics Institute, Inc.
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02140

  Publications

Jamil, T; Frisch, H L; Lerman, L S (1989) Relaxation effects in the gel electrophoresis of DNA in intermittent fields. Biopolymers 28:1413-27
Lerman, L S (1987) Detecting sequence changes in a gene. Somat Cell Mol Genet 13:419-22
Lerman, L S; Silverstein, K (1987) Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis. Methods Enzymol 155:482-501
Lerman, L S; Silverstein, K; Grinfeld, E (1986) Searching for gene defects by denaturing gradient gel electrophoresis. Cold Spring Harb Symp Quant Biol 51 Pt 1:285-97