We will study the photochemistry of polycyclic aromatic amino azides which bind to DNA by intercalation. We will use low temperature Matrix IR, UV-Vis, fluorescence and EPR spectroscopy to identify the specific intermediate(s) produced by photolysis of ethidium azide and proflavine azide. We will photolyze these azides intercalated into mini duplexes of self complementary dinucleotides. The products of these photoreactions will be identified by X-ray crystallography. We will also prepare polyfluorinated derivatives of ethidium azide and proflavine azide. This should greatly simplify the azide photochemistry and increase the absolute reactivity of the photogenerated intermediates. The latter point will increase the chances of covalent attachment in the binding site. We will study these polyfluorinated azides in solution and bound to mini duplexes by laser flash photolysis to obtain the absolute lifetime of the labeling intermediate.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036489-03
Application #
3290559
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1986-12-01
Project End
1991-11-30
Budget Start
1988-12-01
Budget End
1989-11-30
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Ohio State University
Department
Type
Schools of Arts and Sciences
DUNS #
098987217
City
Columbus
State
OH
Country
United States
Zip Code
43210