Abstract

In the next grant year, we will continue to pursue two of our original objectives: (1) to characterize the Ca2 ion-binding properties of calmodulin using Tb3 ion as a fluorescent probe, and (2) to characterize the biological properties of the calcineurin, including its ontogenetic development in rat brain.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM036734-18
Application #
3291207
Study Section
Biochemistry Study Section (BIO)
Project Start
1985-09-18
Project End
1988-08-31
Budget Start
1987-09-01
Budget End
1988-08-31
Support Year
18
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
St. Jude Children's Research Hospital
Department
Type
DUNS #
067717892
City
Memphis
State
TN
Country
United States
Zip Code
38105

  Publications

Huang, S; Cheung, W Y (1994) H+ is involved in the activation of calcineurin by calmodulin. J Biol Chem 269:22067-74
Huang, S; Carlson, G M; Cheung, W Y (1994) Calmodulin-dependent enzymes undergo a protein-induced conformational change that is associated with their interactions with calmodulin. J Biol Chem 269:7631-8
Chao, S H; Bu, C H; Cheung, W Y (1990) Activation of troponin C by Cd2+ and Pb2+. Arch Toxicol 64:490-6
Pezzi, L; Merat, D L; Winkler, M A et al. (1989) Calmodulin-dependent phosphatase preferentially dephosphorylates a 28 kDa protein in human platelets. Int J Biochem 21:791-8
Huang, S L; Merat, D; Cheung, W Y (1989) Phosphatidylinositol modulates the response of calmodulin-dependent phosphatase to calmodulin. Arch Biochem Biophys 270:42-9
Cheung, W Y (1988) Calmodulin and its activation by cadmium ion. Ann N Y Acad Sci 522:74-87
Winkler, M A; Zysk, J R; Cheung, W Y (1987) Production and characterization of a monoclonal antibody cross-reactive with calmodulin, calmodulin-dependent phosphodiesterase, and protein phosphatase. Methods Enzymol 139:505-18
Winkler, M A; Fried, V A; Merat, D L et al. (1987) Differential reactivities of lysines in calmodulin complexed to phosphatase. J Biol Chem 262:15466-71
Merat, D L; Cheung, W Y (1987) Calmodulin-dependent protein phosphatase: isolation of subunits and reconstitution to holoenzyme. Methods Enzymol 139:79-87
Winkler, M A; DeWitt, L M; Cheung, W Y (1987) Calmodulin and calcium channel blockers. Hypertension 9:217-23

Showing the most recent 10 out of 11 publications