The study of the ten""""""""rate bacteriophage has been exceedingly useful in generating information about universal regulatory mechanisms. Regulation of gene expression by a system of transcription termination - antitermination, first described in phage, has now been seen in eukaryotic cells, in particular the control of c-myc and HIV gene expression. The cellular factors, viral proteins, and specific sites required for antitermination are partially understood in the phage lambda system, and almost completely unknown for phage HKO22. The differences and similarities between these two phages, as well as the interactions betwen their regulatory systems, is being explored. The approach is both genetic and biochemical, and involves the isolation of mutants, the overproduction and purification of regulatory proteins, and the construction of mutant sites. Another phage regulatory system, repression, is subject to control by a host function, RpsB (S2). The control is at the level of mRNA translation; the mehanism of this regulation will be determined by analysis of mutants and establishment of an in vitro translation system.
Saxena, Shivalika; Myka, Kamila K; Washburn, Robert et al. (2018) Escherichia coli transcription factor NusG binds to 70S ribosomes. Mol Microbiol 108:495-504 |
Gottesman, Max E; Mustaev, Arkady (2018) Inorganic phosphate, arsenate, and vanadate enhance exonuclease transcript cleavage by RNA polymerase by 2000-fold. Proc Natl Acad Sci U S A 115:2746-2751 |
Zuber, Philipp K; Hahn, Lukas; Reinl, Anne et al. (2018) Structure and nucleic acid binding properties of KOW domains 4 and 6-7 of human transcription elongation factor DSIF. Sci Rep 8:11660 |
Schrank, Benjamin R; Aparicio, Tomas; Li, Yinyin et al. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature 559:61-66 |
Kang, Jin Young; Olinares, Paul Dominic B; Chen, James et al. (2017) Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex. Elife 6: |
Mustaev, Arkady; Roberts, Jeffrey; Gottesman, Max (2017) Transcription elongation. Transcription 8:150-161 |
Strauß, Martin; Vitiello, Christal; Schweimer, Kristian et al. (2016) Transcription is regulated by NusA:NusG interaction. Nucleic Acids Res 44:5971-82 |
Mustaev, Arkady; Vitiello, Christal L; Gottesman, Max E (2016) Probing the structure of Nun transcription arrest factor bound to RNA polymerase. Proc Natl Acad Sci U S A 113:8693-8 |
Washburn, Robert S; Gottesman, Max E (2015) Regulation of transcription elongation and termination. Biomolecules 5:1063-78 |
Vitiello, Christal L; Kireeva, Maria L; Lubkowska, Lucyna et al. (2014) Coliphage HK022 Nun protein inhibits RNA polymerase translocation. Proc Natl Acad Sci U S A 111:E2368-75 |
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