The overall goals of this project are: 1) to develop highly efficient water proton NMR relaxation agents in the form of gadolinium(III) complexes that bind non-covalently to human serum albumin (HSA) and 2) to elucidate the structural features of the complexes that control HSA binding behavior and relaxation efficiency (relaxivity). This basic structure-binding and structure-relaxivity investigation will define a new class of selectively intravascular NMR imaging diagnostic agents for potential human use at much lower doses than current agents. In addition, the HSA-chelate interactions studied in this project will serve as a useful model system to understand the effect of in-vivo binding of agents on the relaxation properties of tissue. A variety of hydrophobic groups will be incorporated into known aminocarboxylate ligands to promote HSA binding. The complexes of Gd3+ or other lanthanide ions (terbium(III), europium(III), prasedymium(III), or ytterbium(III)), will be isolated for HSA binding studies. Equilibrium dialysis and fluorescence techniques will be utilized to characterize the binding behavior; the influence of the structure of the different complexes on the nature of the binding interactions and their affinities will be assessed. Proton NMR spectra and luminescence lifetime measurements will be obtained to assess the structure of the complexes, before and after binding to HSA. The water proton NMR relaxivities of the free and HSA-bound Gd3+ complexes will be measured at multiple magnetic field strength to fully characterize the enhancement effect stemming from HSA binding. Structure-relaxivity comparisons, including the influence of flexibility in the chelate or in its attachment to HSA, the number of metal-coordinated water molecules, and the effective symmetry of the metal center, will be obtained.
| Jenkins, B G; Armstrong, E; Lauffer, R B (1991) Site-specific water proton relaxation enhancement of iron(III) chelates noncovalently bound to human serum albumin. Magn Reson Med 17:164-78 |
| Jenkins, B G; Lauffer, R B (1990) Detection of site-specific binding and co-binding of ligands to human serum albumin using 19F NMR. Mol Pharmacol 37:111-8 |
