Steroidogenic P450 Enzymes - Structural Mutagenesis

Waterman, Michael R.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 2R01GM037942-06
• Application #: 3293804
• Study Section: Physical Biochemistry Study Section (PB)
• Project Start: 1987-04-01
• Project End: 1992-07-17
• Budget Start: 1992-04-01
• Budget End: 1992-07-17
• Support Year: 6
• Fiscal Year: 1992

Institution

• Name: University of Texas Sw Medical Center Dallas
• Type: Schools of Medicine
• City: Dallas
• State: TX
• Country: United States
• Zip Code: 75390

Publications

Oyama, Tsunehiro; Kagawa, Norio; Sugio, Kenji et al. (2009) Expression of aromatase CYP19 and its relationship with parameters in NSCLC. Front Biosci (Landmark Ed) 14:2285-92

Miller, Todd W; Shin, Incheol; Kagawa, Norio et al. (2008) Aromatase is phosphorylated in situ at serine-118. J Steroid Biochem Mol Biol 112:95-101

Tosha, Takehiko; Kagawa, Norio; Arase, Miharu et al. (2008) Interaction between substrate and oxygen ligand responsible for effective O-O bond cleavage in bovine cytochrome P450 steroid 21-hydroxylase proved by Raman spectroscopy. J Biol Chem 283:3708-17

Zollner, Andy; Kagawa, Norio; Waterman, Michael R et al. (2008) Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli. FEBS J 275:799-810

Podust, Larissa M; von Kries, Jens P; Eddine, Ali Nasser et al. (2007) Small-molecule scaffolds for CYP51 inhibitors identified by high-throughput screening and defined by X-ray crystallography. Antimicrob Agents Chemother 51:3915-23

Lamb, David C; Guengerich, F Peter; Kelly, Steven L et al. (2006) Exploiting Streptomyces coelicolor A3(2) P450s as a model for application in drug discovery. Expert Opin Drug Metab Toxicol 2:27-40

Lamb, David C; Kim, Youngchang; Yermalitskaya, Liudmila V et al. (2006) A second FMN binding site in yeast NADPH-cytochrome P450 reductase suggests a mechanism of electron transfer by diflavin reductases. Structure 14:51-61

Sherman, David H; Li, Shengying; Yermalitskaya, Liudmila V et al. (2006) The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae. J Biol Chem 281:26289-97

Tosha, Takehiko; Kagawa, Norio; Ohta, Takehiro et al. (2006) Raman evidence for specific substrate-induced structural changes in the heme pocket of human cytochrome P450 aromatase during the three consecutive oxygen activation steps. Biochemistry 45:5631-40

Arase, Miharu; Waterman, Michael R; Kagawa, Norio (2006) Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli. Biochem Biophys Res Commun 344:400-5