Thy-1 is a membrane glycoprotein expressed predominantly on T lymphoyctes and neuronal cells. Because of its structural homology to immunoglobulin domains, it has been proposed that Thy-1 may function as a receptor on T cells. Indeed, several studies have demonstrated that antibodies directed against Thy-1 can stimulate T cells to proliferate and produce lymphokines. In contrast to other membrane proteins involved in regulation of the immune system, Thy-1 appears to be anchored to the membrane via a glycophospholipid which replaces a 31 amino acid segment at the carboxyl terminus of newly synthesized Thy-1. Mutant and chimeric Thy-1 genes will be constructed and used in transient and stable transfection systems to investigate the mechanism of this conversion and the role of the glycolipid anchor in membrane """"""""trafficking"""""""" and T cell activation.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM038207-02
Application #
3294370
Study Section
Immunobiology Study Section (IMB)
Project Start
1987-01-01
Project End
1988-12-31
Budget Start
1988-07-01
Budget End
1988-12-31
Support Year
2
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Hospital for Joint Diseases Ortho Institute
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10003
Tokugawa, Y; Koyama, M; Silver, J (1997) A molecular basis for species differences in Thy-1 expression patterns. Mol Immunol 34:1263-72