Abstract

Time resolved circular dichroism spectroscopy is a technique that we have developed in our laboratory for the study of protein conformational dynamics. The capabilities of our experimental approach allows one to measure conformational motions of proteins that occur on the picosecond or slower time scale. In this proposal, we discuss, in detail, the experimental approach for measuring time resolved circular dichroism spectra on the picosecond time scale and applications of our technique to the study of protein conformation changes caused by the photoinduced debinding of substrates bound to myoglobin, hemoglobin and model systems. In addition, we discuss future applications of our new experimental technique to determine (1) protein motions induced by the isomerization of retinal in the visual protein rhodopsin, and (2) changes in protein structure caused by electron transfer chemistry in hybrid hemoglobins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM041942-03
Application #
3300446
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Project Start
1990-01-01
Project End
1993-06-30
Budget Start
1992-01-01
Budget End
1993-06-30
Support Year
3
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
University of California San Diego
Department
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093