Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM043149-03S1
Application #
3302110
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1990-07-01
Project End
1995-06-30
Budget Start
1992-07-01
Budget End
1993-06-30
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Chadsey, M S; Hughes, K T (2001) A multipartite interaction between Salmonella transcription factor sigma28 and its anti-sigma factor FlgM: implications for sigma28 holoenzyme destabilization through stepwise binding. J Mol Biol 306:915-29
Karlinsey, J E; Lonner, J; Brown, K L et al. (2000) Translation/secretion coupling by type III secretion systems. Cell 102:487-97
Chadsey, M S; Karlinsey, J E; Hughes, K T (1998) The flagellar anti-sigma factor FlgM actively dissociates Salmonella typhimurium sigma28 RNA polymerase holoenzyme. Genes Dev 12:3123-36
Nanassy, O Z; Hughes, K T (1998) In vivo identification of intermediate stages of the DNA inversion reaction catalyzed by the Salmonella Hin recombinase. Genetics 149:1649-63
Chilcott, G S; Hughes, K T (1998) The type III secretion determinants of the flagellar anti-transcription factor, FlgM, extend from the amino-terminus into the anti-sigma28 domain. Mol Microbiol 30:1029-40
Karlinsey, J E; Pease, A J; Winkler, M E et al. (1997) The flk gene of Salmonella typhimurium couples flagellar P- and L-ring assembly to flagellar morphogenesis. J Bacteriol 179:2389-400
Adams, C W; Nanassy, O; Johnson, R C et al. (1997) Role of arginine-43 and arginine-69 of the Hin recombinase catalytic domain in the binding of Hin to the hix DNA recombination sites. Mol Microbiol 24:1235-47
Gillen, K L; Hughes, K T (1993) Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM. J Bacteriol 175:7006-15
Hughes, K T; Gillen, K L; Semon, M J et al. (1993) Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator. Science 262:1277-80
Hughes, K T; Gaines, P C; Karlinsey, J E et al. (1992) Sequence-specific interaction of the Salmonella Hin recombinase in both major and minor grooves of DNA. EMBO J 11:2695-705

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