Abstract

The goal of this study is the use of misacylated transfer RNA's in vitro protein biosynthesizing systems to . define the structure features in the aminoacyl moiety that permit its participation in the peptidyltransferase reaction . probe the physiochemical nature of the peptidyltransferase site . effect the elaboration of structurally modified enzymes containing synthetic amino acids at single, predetermined sites

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM043328-01
Application #
3302385
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1990-03-01
Project End
1995-02-28
Budget Start
1990-03-01
Budget End
1991-02-28
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
Schools of Arts and Sciences
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Karginov, V A; Mamaev, S V; Hecht, S M (1997) In vitro suppression as a tool for the investigation of translation initiation. Nucleic Acids Res 25:3912-6
Resto, E; Iida, A; Van Cleve, M D et al. (1992) Amplification of protein expression in a cell free system. Nucleic Acids Res 20:5979-83