This project deals with the mechanisms that account for regulation of ribosomal RNA synthesis. The mammalian rDNA promoter consists of two elements: a core promoter (CPE) and an upstream element (UPE). These interact with at least two transcription factors: UBF and SL1. SL1 binds both UPE and CPE and is required for transcription in vitro. UBF binds the CPE and is necessary for maximum transcriptional activity in vitro. The working hypothesis holds that UBF bends the DNA so as to approximate the SF1 binding sites. Rb blocks activation by UBF and prevents interaction between UBF and SL1. The expression of UBF is regulated by growth stimulation and differentiation, and the activity of UBF is regulated by phosphorylation. This project focuses primarily on the regulation of UBF expression and activity and the role of these processes in regulation of rDNA transcription.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
7R01GM046991-06
Application #
2444814
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1992-02-01
Project End
2000-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
6
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033
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Hirschler-Laszkiewicz, I; Cavanaugh, A; Hu, Q et al. (2001) The role of acetylation in rDNA transcription. Nucleic Acids Res 29:4114-24

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