This project deals with the mechanisms that account for regulation of ribosomal RNA synthesis. The mammalian rDNA promoter consists of two elements: a core promoter (CPE) and an upstream element (UPE). These interact with at least two transcription factors: UBF and SL1. SL1 binds both UPE and CPE and is required for transcription in vitro. UBF binds the CPE and is necessary for maximum transcriptional activity in vitro. The working hypothesis holds that UBF bends the DNA so as to approximate the SF1 binding sites. Rb blocks activation by UBF and prevents interaction between UBF and SL1. The expression of UBF is regulated by growth stimulation and differentiation, and the activity of UBF is regulated by phosphorylation. This project focuses primarily on the regulation of UBF expression and activity and the role of these processes in regulation of rDNA transcription.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM046991-07
Application #
2734723
Study Section
Molecular Cytology Study Section (CTY)
Program Officer
Tompkins, Laurie
Project Start
1992-02-01
Project End
2000-06-30
Budget Start
1998-07-01
Budget End
1999-06-30
Support Year
7
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033
Zhang, Xue-Qian; Qureshi, Anwer; Song, Jianliang et al. (2003) Phospholemman modulates Na+/Ca2+ exchange in adult rat cardiac myocytes. Am J Physiol Heart Circ Physiol 284:H225-33
Song, Jianliang; Zhang, Xue-Qian; Carl, Lois L et al. (2002) Overexpression of phospholemman alters contractility and [Ca(2+)](i) transients in adult rat myocytes. Am J Physiol Heart Circ Physiol 283:H576-83
Zhang, Xue-Qian; Song, Jianliang; Qureshi, Anwer et al. (2002) Rescue of contractile abnormalities by Na+/Ca2+ exchanger overexpression in postinfarction rat myocytes. J Appl Physiol 93:1925-31
Tadros, George M; Zhang, Xue-Qian; Song, Jianliang et al. (2002) Effects of Na(+)/Ca(2+) exchanger downregulation on contractility and [Ca(2+)](i) transients in adult rat myocytes. Am J Physiol Heart Circ Physiol 283:H1616-26
Chu, Xin; Cheung, Joseph Y; Barber, Dwayne L et al. (2002) Erythropoietin modulates calcium influx through TRPC2. J Biol Chem 277:34375-82
Flynn, Brian J; Mian, Hamayun S; Cera, Peter J et al. (2002) Early molecular changes in bladder hypertrophy due to bladder outlet obstruction. Urology 59:978-82
Stefanovsky, V Y; Pelletier, G; Hannan, R et al. (2001) An immediate response of ribosomal transcription to growth factor stimulation in mammals is mediated by ERK phosphorylation of UBF. Mol Cell 8:1063-73
Zhang, X Q; Song, J; Rothblum, L I et al. (2001) Overexpression of Na+/Ca2+ exchanger alters contractility and SR Ca2+ content in adult rat myocytes. Am J Physiol Heart Circ Physiol 281:H2079-88
Biggiogera, M; Malatesta, M; Abolhassani-Dadras, S et al. (2001) Revealing the unseen: the organizer region of the nucleolus. J Cell Sci 114:3199-205
Hirschler-Laszkiewicz, I; Cavanaugh, A; Hu, Q et al. (2001) The role of acetylation in rDNA transcription. Nucleic Acids Res 29:4114-24

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