The long-term goal of this laboratory is to formulate an understanding of the molecular mechanisms operating to regulate expression of class II genes. This will be accomplished through the characterization of the proteins involved, and their various protein-DNA and protein-protein interactions. To this end, the overall aim of this proposal is to continue and expand studies now in progress on a novel heterodimeric factor, Dr1/DRAP1, that interacts with a key component of the RNA polymerase II transcription machinery. This interaction results in repression of class II gene expression. Regulation of gene expression is a complex process that can be achieved at multiple steps. Studies have demonstrated that initiation of transcription is a primary site for regulation. The first step during formation of a transcription competent complex is the binding of TFIID to the TATA motif, providing a recognition site for the association of RNA polymerase II and auxiliary proteins. TFIID also interacts with activator proteins. A great deal of effort has been directed towards the isolation of proteins that activate transcription. However, an equally important mechanism for regulating gene expression is repression. Studies carried out during the past years have uncovered a mechanism for repression involving protein:protein interactions.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM048518-07
Application #
2838598
Study Section
Molecular Biology Study Section (MBY)
Program Officer
Tompkins, Laurie
Project Start
1992-12-01
Project End
2000-11-30
Budget Start
1998-12-01
Budget End
1999-11-30
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Biochemistry
Type
Schools of Medicine
DUNS #
622146454
City
Piscataway
State
NJ
Country
United States
Zip Code
08854
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