Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
1R01GM051831-01A2
Application #
2190573
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1996-05-01
Project End
1999-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Biochemistry
Type
Schools of Medicine
DUNS #
073134603
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Brown, Nina M; Kennedy, M Claire; Antholine, William E et al. (2002) Detection of a [3Fe-4S] cluster intermediate of cytosolic aconitase in yeast expressing iron regulatory protein 1. Insights into the mechanism of Fe-S cluster cycling. J Biol Chem 277:7246-54
Vasquez-Vivar, J; Kalyanaraman, B; Kennedy, M C (2000) Mitochondrial aconitase is a source of hydroxyl radical. An electron spin resonance investigation. J Biol Chem 275:14064-9
Konorev, E A; Zhang, H; Joseph, J et al. (2000) Bicarbonate exacerbates oxidative injury induced by antitumor antibiotic doxorubicin in cardiomyocytes. Am J Physiol Heart Circ Physiol 279:H2424-30
Konorev, E A; Kennedy, M C; Kalyanaraman, B (1999) Cell-permeable superoxide dismutase and glutathione peroxidase mimetics afford superior protection against doxorubicin-induced cardiotoxicity: the role of reactive oxygen and nitrogen intermediates. Arch Biochem Biophys 368:421-8
Brown, N M; Anderson, S A; Steffen, D W et al. (1998) Novel role of phosphorylation in Fe-S cluster stability revealed by phosphomimetic mutations at Ser-138 of iron regulatory protein 1. Proc Natl Acad Sci U S A 95:15235-40
Schalinske, K L; Anderson, S A; Tuazon, P T et al. (1997) The iron-sulfur cluster of iron regulatory protein 1 modulates the accessibility of RNA binding and phosphorylation sites. Biochemistry 36:3950-8
Costello, L C; Liu, Y; Franklin, R B et al. (1997) Zinc inhibition of mitochondrial aconitase and its importance in citrate metabolism of prostate epithelial cells. J Biol Chem 272:28875-81
Kennedy, M C; Antholine, W E; Beinert, H (1997) An EPR investigation of the products of the reaction of cytosolic and mitochondrial aconitases with nitric oxide. J Biol Chem 272:20340-7
Lauble, H; Kennedy, M C; Emptage, M H et al. (1996) The reaction of fluorocitrate with aconitase and the crystal structure of the enzyme-inhibitor complex. Proc Natl Acad Sci U S A 93:13699-703