proposal): The proposal suggests that RPA, a multisubunit (p70, p34 and p11) protein, which is involved in DNA replication and DNA repair, may be a target for regulation. This stems from the fact that the amount of RPA subunits does not vary during the cell cycle. Hence, the DNA replication and DNA repair activities of RPA could be regulated by either protein assembly or post- translational modification. The investigator proposes to test whether the assembly of the RPA complex is regulated during the cell cycle. In addition, he will examine whether RPA regulates the formation of initiation complexes at the SV40 origin of replication. Since phosphorylation of RPA could have a role in regulating its activities, the investigator will map the phosphorylation sites and will characterize the kinases in this phosphorylation. RPA phosphorylation sites will be mutated and mutant RPA will be analyzed along with the wild type RPA in a coupled repair- replication system, which was recently developed in the investigator's laboratory.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM052358-04
Application #
2668496
Study Section
Molecular Biology Study Section (MBY)
Project Start
1996-03-01
Project End
2000-02-29
Budget Start
1998-03-01
Budget End
1999-02-28
Support Year
4
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Indiana University-Purdue University at Indianapolis
Department
Biochemistry
Type
Schools of Medicine
DUNS #
005436803
City
Indianapolis
State
IN
Country
United States
Zip Code
46202