description): Many different RNA binding and catalytic activities can be isolated from pools of random sequences, and recent work suggests that the same will be true of proteins. It is proposed to use in vitro selection and directed evolution to explore ways for generating new molecular functions de novo from random sequences and by modification of existing functions. The relationship between information content and functional activity will be examined to gauge the difficulty of obtaining progressively more active molecules. The re-use of previously selected functions to evolve new functions will also be studied. The idea that proteins are better than RNA at evolving new functions from existing functions will also be tested.
| Carothers, James M; Oestreich, Stephanie C; Szostak, Jack W (2006) Aptamers selected for higher-affinity binding are not more specific for the target ligand. J Am Chem Soc 128:7929-37 |
| Salehi-Ashtiani, Kourosh; Luptak, Andrej; Litovchick, Alexander et al. (2006) A genomewide search for ribozymes reveals an HDV-like sequence in the human CPEB3 gene. Science 313:1788-92 |
| Carothers, James M; Davis, Jonathan H; Chou, James J et al. (2006) Solution structure of an informationally complex high-affinity RNA aptamer to GTP. RNA 12:567-79 |
| Ichida, Justin K; Zou, Keyong; Horhota, Allen et al. (2005) An in vitro selection system for TNA. J Am Chem Soc 127:2802-3 |
| Shen, Xinchun; Valencia, C Alexander; Szostak, Jack W et al. (2005) Scanning the human proteome for calmodulin-binding proteins. Proc Natl Acad Sci U S A 102:5969-74 |
| Plummer, Kelly A; Carothers, James M; Yoshimura, Masahiro et al. (2005) In vitro selection of RNA aptamers against a composite small molecule-protein surface. Nucleic Acids Res 33:5602-10 |
| Zou, Keyong; Horhota, Allen; Yu, Biao et al. (2005) Synthesis of alpha-L-threofuranosyl nucleoside triphosphates (tNTPs). Org Lett 7:1485-7 |
| Horhota, Allen; Zou, Keyong; Ichida, Justin K et al. (2005) Kinetic analysis of an efficient DNA-dependent TNA polymerase. J Am Chem Soc 127:7427-34 |
| Ichida, Justin K; Horhota, Allen; Zou, Keyong et al. (2005) High fidelity TNA synthesis by Therminator polymerase. Nucleic Acids Res 33:5219-25 |
| Sazani, Peter L; Larralde, Rosa; Szostak, Jack W (2004) A small aptamer with strong and specific recognition of the triphosphate of ATP. J Am Chem Soc 126:8370-1 |
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