Abstract

The central hypothesis of the proposal is that PLDs are important regulators of cell biological processes. More specifically, the P.I. proposes that PLD1 regulates secretion in response to stimulation and that PLD2 plays a role in receptor-mediated recycling or secretion.
The specific aims address the central hypothesis.
Aims 1 and 3 deal mainly with the molecular biology, enzymology, and activation of the PLDs, while Aims 2 and 4 deal more directly with the functions of the PLDs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM054813-02
Application #
2857249
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1998-01-01
Project End
2001-12-31
Budget Start
1999-01-01
Budget End
1999-12-31
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
State University New York Stony Brook
Department
Pharmacology
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794

  Publications

Kraft, Catherine A; Garrido, Jose Luis; Fluharty, Eric et al. (2008) Role of phosphatidic acid in the coupling of the ERK cascade. J Biol Chem 283:36636-45
Watanabe, Hiroshi; Yamazaki, Masakazu; Miyazaki, Hideyuki et al. (2004) Phospholipase D2 functions as a downstream signaling molecule of MAP kinase pathway in L1-stimulated neurite outgrowth of cerebellar granule neurons. J Neurochem 89:142-51
Du, Guangwei; Altshuller, Yelena M; Vitale, Nicolas et al. (2003) Regulation of phospholipase D1 subcellular cycling through coordination of multiple membrane association motifs. J Cell Biol 162:305-15
Huang, Ping; Frohman, Michael A (2003) The role of phospholipase D in Glut-4 translocation. Diabetes Metab Res Rev 19:456-63
Du, Guangwei; Morris, Andrew J; Sciorra, Vicki A et al. (2002) G-protein-coupled receptor regulation of phospholipase D. Methods Enzymol 345:265-74
Yamazaki, Masakazu; Miyazaki, Hideyuki; Watanabe, Hiroshi et al. (2002) Phosphatidylinositol 4-phosphate 5-kinase is essential for ROCK-mediated neurite remodeling. J Biol Chem 277:17226-30
Humeau, Y; Vitale, N; Chasserot-Golaz, S et al. (2001) A role for phospholipase D1 in neurotransmitter release. Proc Natl Acad Sci U S A 98:15300-5
Rizzo, M A; Kraft, C A; Watkins, S C et al. (2001) Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade. J Biol Chem 276:34928-33
Du, G; Altshuller, Y M; Kim, Y et al. (2000) Dual requirement for rho and protein kinase C in direct activation of phospholipase D1 through G protein-coupled receptor signaling. Mol Biol Cell 11:4359-68
Park, J B; Kim, J H; Kim, Y et al. (2000) Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha-actinin in an ADP-ribosylation factor-reversible manner. J Biol Chem 275:21295-301

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