Despite the critical role mechanical stimuli play in hearing and touch, the molecular basis of mechanosensation in vertebrates remains elusive. The proposed research uses genetic, cellular, and molecular techniques in C. elegans to identify receptor proteins, signal transduction pathways and synaptic pathways involved in the detection of noxious mechanical and chemical stimuli. The C. elegans ASH neurons respond to nose touch, high osmolarity, and volatile repellents. Mutations have been identified which perturb responses to specific ASH stimuli. The genes involved will be molecularly characterized. They will address the role of the OSM-10 protein in osmosensation, and examine newly identified peptidergic neurotransmitters in the ASH circuit.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM057918-02
Application #
6180782
Study Section
Special Emphasis Panel (ZRG1-IFCN-4 (01))
Program Officer
Baughman, Robert W
Project Start
1999-05-01
Project End
2004-04-30
Budget Start
2000-05-01
Budget End
2001-04-30
Support Year
2
Fiscal Year
2000
Total Cost
$329,868
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
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