Single Molecule Studies of DNA Helicases

Bianco, Piero R.

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: Research Project (R01)
• Project #: 5R01GM066831-02
• Application #: 6692214
• Study Section: Biochemistry Study Section (BIO)
• Program Officer: Lewis, Catherine D
• Project Start: 2003-01-01
• Project End: 2007-12-31
• Budget Start: 2004-01-01
• Budget End: 2004-12-31
• Support Year: 2
• Fiscal Year: 2004
• Total Cost: $261,972

Institution

• Name: State University of New York at Buffalo
• Department: Microbiology/Immun/Virology
• Type: Schools of Medicine
• DUNS #: 038633251
• City: Buffalo
• State: NY
• Country: United States
• Zip Code: 14260

Publications

Yüksel, Deniz; Bianco, Piero R; Kumar, Krishna (2016) De novo design of protein mimics of B-DNA. Mol Biosyst 12:169-77

Bianco, Piero R; Webb, Martin R (2012) Helicase unwinding: active or merely perfect? J Mol Biol 420:139-40

Bianco, Piero R; Stanenas, Adam J; Liu, Juan et al. (2012) Fluorescent single-stranded DNA-binding proteins enable in vitro and in vivo studies. Methods Mol Biol 922:235-44

Bianco, Piero R; Xu, Cuiling; Chi, Min (2009) Type I restriction endonucleases are true catalytic enzymes. Nucleic Acids Res 37:3377-90

Brewer, Laurence R; Bianco, Piero R (2008) Laminar flow cells for single-molecule studies of DNA-protein interactions. Nat Methods 5:517-25

Buss, Jackson A; Kimura, Yuji; Bianco, Piero R (2008) RecG interacts directly with SSB: implications for stalled replication fork regression. Nucleic Acids Res 36:7029-42

Bianco, Piero R; Bradfield, Justin J; Castanza, Lauren R et al. (2007) Rad54 oligomers translocate and cross-bridge double-stranded DNA to stimulate synapsis. J Mol Biol 374:618-40

Slocum, Stephen L; Buss, Jackson A; Kimura, Yuji et al. (2007) Characterization of the ATPase activity of the Escherichia coli RecG protein reveals that the preferred cofactor is negatively supercoiled DNA. J Mol Biol 367:647-64

Anand, Syam P; Zheng, Haocheng; Bianco, Piero R et al. (2007) DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange. J Bacteriol 189:4502-9

Kimura, Yuji; Bianco, Piero R (2006) Single molecule studies of DNA binding proteins using optical tweezers. Analyst 131:868-74