Cytoplasmic dynein is a molecular motor crucially involved in many processes essential for correct neurological function. Its improper function causes failed neurodevelopment (e.g. Miller- Dieker Lissencephaly), neurodegeneration, and other cognitive diseases such as schizophrenia. The wide range of dynein roles is made possible by tuning its function with accessory proteins such as Lis1, NudE, and NudEL. However, while genetic studies, both via mutagenesis in model organisms, and via identification of causative/risk factors in human disease, have repeatedly established that these proteins are in the dynein pathway and are important for dynein function-that is, dynein-mediated transport is impaired when their function is lost-mechanistically it has been impossible to determine what they do, or why they are important. Lacking such knowledge makes understanding disease progression difficult, as well as making it impossible to rationally design therapeutic approaches to correct the impairments. Our work determines at a mechanistic level exactly how the different cofactors alter dynein function, and starts to determine the ways that these co-factors effects on dynein are themselves regulated. At a mechanistic level, we will better understand how dynein is turned off by NudE, and then re-activated by Lis1. At a global level, we will determine functionally what NudEL does to dynein function (if anything), and how the function of NudE, NudEL, and Lis1 are altered by phosphorylation. Since dynein is crucial for many aspects of neuronal function, this will importantly advance the general field of neurobiology, and possibly allow design of more targeted therapeutic approaches.
Dynein-based transport is directly related to public health: viruses such as herpes and adenovirus employ dynein to spread through cells and important cargos like mitochondria and endosomes are positioned by dynein. Altered dynein function is linked to neurodegeneration. Mutations in the protein Lis1 studied here are linked to the human neurodevelopmental disease Miller-Dieker Lissencephaly.
|Shojania Feizabadi, Mitra; Janakaloti Narayanareddy, Babu Reddy; Vadpey, Omid et al. (2015) Microtubule C-Terminal Tails Can Change Characteristics of Motor Force Production. Traffic 16:1075-87|
|Tripathy, Suvranta K; Weil, Sarah J; Chen, Chen et al. (2014) Autoregulatory mechanism for dynactin control of processive and diffusive dynein transport. Nat Cell Biol 16:1192-201|
|Jun, Yonggun; Tripathy, Suvranta K; Narayanareddy, Babu R J et al. (2014) Calibration of optical tweezers for in vivo force measurements: how do different approaches compare? Biophys J 107:1474-84|
|Sigua, Robilyn; Tripathy, Suvranta; Anand, Preetha et al. (2012) Isolation and purification of kinesin from Drosophila embryos. J Vis Exp :|
|Xu, Jing; Shu, Zhanyong; King, Stephen J et al. (2012) Tuning multiple motor travel via single motor velocity. Traffic 13:1198-205|
|Yi, Julie Y; Ori-McKenney, Kassandra M; McKenney, Richard J et al. (2011) High-resolution imaging reveals indirect coordination of opposite motors and a role for LIS1 in high-load axonal transport. J Cell Biol 195:193-201|
|McKenney, Richard J; Vershinin, Michael; Kunwar, Ambarish et al. (2010) LIS1 and NudE induce a persistent dynein force-producing state. Cell 141:304-14|
|Ori-McKenney, Kassandra M; Xu, Jing; Gross, Steven P et al. (2010) A cytoplasmic dynein tail mutation impairs motor processivity. Nat Cell Biol 12:1228-34|
|Ziebert, F; Vershinin, M; Gross, S P et al. (2009) Collective alignment of polar filaments by molecular motors. Eur Phys J E Soft Matter 28:401-9|
|Kunwar, Ambarish; Vershinin, Michael; Xu, Jing et al. (2008) Stepping, strain gating, and an unexpected force-velocity curve for multiple-motor-based transport. Curr Biol 18:1173-83|
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