We propose to develop a mass spectrometry (MS) analysis system and a protocol that can be applied for chemical analysis at the point of care (POC) for medical diagnosis.
The aims are to develop (1) a miniature mass spectrometer that has compact size, low cost of ownership and maintenance but with adequate performance for quantitative analysis, and (2) sample cartridges for direct analysis of blood samples and (3) an operational protocol that allows this system to be used by nurses and physicians, who do not need to have special training in chemical analysis using MS. This research team at Purdue University has been consistently pushing the development of technologies and applications to allow MS analysis to be used for POC tests at clinical sites and ultimately for personal health care. Our long term goal is to develop a POC MS system and collaborate with researchers such as those at The University of Texas MD Anderson Cancer Center (UTMDACC) to validate the technology and to incorporate it into drug development to optimize disease therapy. The critical issues in the development of a POC MS system have been identified as the miniaturization of the entire analysis system and the simplification of the analysis protocol. In this R01 project, we propose to develop a complete POC MS analysis system and associated operational protocol based on miniature ion trap and paper spray technologies, and to validate the system for therapeutic drug monitoring with oncology drugs. The expected outcome from the proposed work is the generation of the first POC MS system with a relatively complete solution for therapeutic drug monitoring and with potential for a significant impact on future development of MS-based chemical analysis systems for clinical diagnosis.

Public Health Relevance

The proposed research has the potential to lead to the development of a first point-of- care mass spectrometry system for direct analysis of raw biological samples. It has strong relevance to general public health by providing a powerful tool for fast, easy, highly sensitive, selective and quantitative chemical monitoring for clinical diagnosis or personal health care.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Instrumentation and Systems Development Study Section (ISD)
Program Officer
Friedman, Fred K
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Purdue University
Engineering (All Types)
Schools of Engineering
West Lafayette
United States
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D'Hue, Cedric; Moore, Michael; Summerlin, Don-John et al. (2018) Feasibility of desorption electrospray ionization mass spectrometry for diagnosis of oral tongue squamous cell carcinoma. Rapid Commun Mass Spectrom 32:133-141
Pu, Fan; Zhang, Wenpeng; Bateman, Kevin P et al. (2017) Using miniature MS system with automatic blood sampler for preclinical pharmacokinetics study. Bioanalysis 9:1633-1641
Pu, Fan; Zhang, Wenpeng; Han, Chao et al. (2017) Fast Quantitation of Pyrazole Fungicides in Wine by Ambient Ionization Mass Spectrometry. Anal Methods 9:5058-5064
Zhou, Xiaoyu; Ouyang, Zheng (2016) Following the Ions through a Mass Spectrometer with Atmospheric Pressure Interface: Simulation of Complete Ion Trajectories from Ion Source to Mass Analyzer. Anal Chem 88:7033-40
Ma, Xiaoxiao; Ouyang, Zheng (2016) Ambient ionization and miniature mass spectrometry system for chemical and biological analysis. Trends Analyt Chem 85:10-19
Ferreira, Christina R; Yannell, Karen E; Jarmusch, Alan K et al. (2016) Ambient Ionization Mass Spectrometry for Point-of-Care Diagnostics and Other Clinical Measurements. Clin Chem 62:99-110
Du, Y Melodie; Hu, Ye; Xia, Yu et al. (2016) Power Normalization for Mass Spectrometry Data Analysis and Analytical Method Assessment. Anal Chem 88:3156-63
Ma, Xiaoxiao; Chong, Leelyn; Tian, Ran et al. (2016) Identification and quantitation of lipid C=C location isomers: A shotgun lipidomics approach enabled by photochemical reaction. Proc Natl Acad Sci U S A 113:2573-8
Ren, Yue; Chiang, Spencer; Zhang, Wenpeng et al. (2016) Paper-capillary spray for direct mass spectrometry analysis of biofluid samples. Anal Bioanal Chem 408:1385-90
Zhou, Xiaoyu; Ouyang, Zheng (2016) Ion transfer between ion source and mass spectrometer inlet: electro-hydrodynamic simulation and experimental validation. Rapid Commun Mass Spectrom 30 Suppl 1:29-33

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