Long term goals of this proposed study concerns the biochemical endocrinology of gonadotropins. The immediate objective is to determine which subunit of follitropin binds to the hormone receptor and identify and characterize the follitropin receptor in the following steps. 1) To prepare photoaffinity derivatives fo follitropin. 2) To test the binding activity and steroidogenesis of the follitropin derivatives. 3) To covalently label the hormone receptor with the follitropin derivatives. 4) To analyze photoaffinity labeled receptor-follitropin complexes by SDS-PAGE, gel permeation and centrifugation. 5) Characterize receptor components of the complexes by proteolytic digestions, immunoprecipitation.
| Ji, T H; Shin, J (1989) Photoaffinity labeling of the follitropin receptor. Pharmacol Ther 43:345-62 |
| Shin, J; Ji, I; Ji, T H (1986) Structural homologies in the lutropin/human choriogonadotropin receptor and the follitropin receptor on porcine granulosa cells. Biochemistry 25:3410-5 |
| Shin, J; Ji, T H (1985) Intersubunit disulfides of the follitropin receptor. J Biol Chem 260:12828-31 |
| Shin, J; Ji, T H (1985) Composition of cross-linked 125I-follitropin-receptor complexes. J Biol Chem 260:12822-7 |
| Harmatz, D; Ji, T H; Middaugh, C R (1985) Aggregation state of the gonadotropin receptor. Biochem Biophys Res Commun 127:687-92 |
| Shin, J; Ji, T H (1985) Photoaffinity labeling of the follitropin receptor. J Biol Chem 260:14020-5 |
