The long-term objectives of this proposal are 1) to understand the mechanism by which tissue interactions regulate morphogenesis, histogenesis and biochemical differentiation (keratinization) during the development of the vertebrate integument, and (2) to understand how the epidermal basal cell populations (stem cells) of the various skin appendages become committed to their tissue-specific forms of histogenesis and keratin gene expression.
The specific aims are 1) to produce polyclonal and monoclonal antibodies which specifically recognize (in tissues and on immunoblots) the individual alpha and beta keratin polypeptides of the avian integument separated by 2-dimensional gel electrophoresis, 2) to define the sequence in which keratins are expressed (by 2-d gel electrophoresis, and indirect immunofluorescence) during the development of the scutate scales, reticulate scales, feathers and general body skin from normal chicken embryos and embryos which have genetically based skin disorders, 3) to correlate the appearance of individual keratin polypeptides with the fine structural events occurring during epidermal histogenesis of the various skin appendages, 4) to determine if dermal tissues induce the same sequences of fine structural events and the same keratins when they are recombined with foreign epithelia, such as the chorionic epithelium or scaleless epidermis, 5) to determine if presumptive scutate scale dermis can acquire the ability to induce a Beta Stratum in the absence of scutate scale epidermis, and, finally, 6) to determine when the embryonic scutate scale epidermis no longer requires scutate scale dermis in order to elaborate a Beta Stratum. These studies are of particular importance to the understanding and control of the numerous skin disorders, affecting the human population.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD018129-06
Application #
3315116
Study Section
Human Embryology and Development Subcommittee 2 (HED)
Project Start
1983-07-01
Project End
1991-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
6
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of South Carolina at Columbia
Department
Type
Schools of Arts and Sciences
DUNS #
111310249
City
Columbia
State
SC
Country
United States
Zip Code
29208
Shames, R B; Bade, B C; Sawyer, R H (1994) Role of epidermal-dermal tissue interactions in regulating tenascin expression during development of the chick scutate scale. J Exp Zool 269:349-66
Knapp, L W; Shames, R B; Barnes, G L et al. (1993) Region-specific patterns of beta keratin expression during avian skin development. Dev Dyn 196:283-90
Zeltinger, J; Sawyer, R H (1992) Avian scale development. XVII: The epidermis of the scaleless (sc/sc) anterior metatarsal skin is determined, but the dermis lacks permissive cues for the patterned expression of the determined state. Dev Dyn 193:58-69
Zeltinger, J; Sawyer, R H (1992) Avian scale development. XVI. Epidermal commitment to terminal differentiation is prior to definitive scale ridge formation. Dev Biol 149:55-65
Shames, R B; Jennings, A G; Sawyer, R H (1991) Expression of the cell adhesion molecules, L-CAM and N-CAM during avian scale development. J Exp Zool 257:195-207
Zeltinger, J; Sawyer, R H (1991) Avian scale development. XIII. Epidermal germinative cells are committed to appendage-specific differentiation and respond to patterned cues in the dermis. Dev Biol 144:335-52
Shames, R B; Jennings, A G; Sawyer, R H (1991) The initial expression and patterned appearance of tenascin in scutate scales is absent from the dermis of the scaleless (sc/sc) chicken. Dev Biol 147:174-86
Knapp, L W; Linser, P J; Carver, W E et al. (1991) Biochemical identification and immunological localization of two non-keratin polypeptides associated with the terminal differentiation of avian scale epidermis. Cell Tissue Res 265:535-45
Knapp, L W; Dawson, W D (1991) Morphological analysis of hair in the hr-2 mutant deer mouse (Peromyscus maniculatus). J Hered 82:431-4
Shames, R B; Knapp, L W; Carver, W E et al. (1991) Region-specific expression of scutate scale type beta keratins in the developing chick beak. J Exp Zool 260:258-66

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