The general objective is to study the roles of transcriptional regulatory sequences in the control of gene expression during embryonic development and differentiation. The specific objectives are to identify and characterize transcriptional regulatory sequences associated with histone genes of Xenopus laevis and to identify and characterize the putative protein factors that interact with the regulatory sequences. Conventional in vitro nucleic acid manipulation methods will be used to construct mutant histone genes containing deletions, insertions, and rearrangements. Manipulated genes will be microinjected into Xenopus oocytes and their expression will be studied by electrophoresis of radiolabeled RNA. In vivo and in vitro assays will be set up for putative regulatory sequence-binding proteins. These assays will be used to examine the interaction of proteins with specific RNA sequences and to characterize the proteins involved.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD022025-02
Application #
3321260
Study Section
Molecular Biology Study Section (MBY)
Project Start
1986-08-01
Project End
1989-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030