The primary hypothesis under investigation is that humoral and cellular immune responses will affect the biological properties and genetic characteristics of HIV and alter disease progression. The proposed studies will utilize available specimens from a cohort followed longitudinally since 6 months of age or earlier, and focusing primarily on slow progressors and rapid progressors.
The Specific Aims of this application are: (1) to study the natural history of humoral immune responses through quantitation of binding antibodies to core and envelope, quantitation of autologous and heterologous neutralizing antibody, and detection of ADCC; (2) to determine the role of cellular immunity in disease progression through characterization of T cell receptor utilization and CTL; (3) to study the biological properties of virus over time as it relates to disease progression by determining viral burden, host cell range and syncytia production.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD034337-05
Application #
6164912
Study Section
Special Emphasis Panel (SRC (64))
Program Officer
Nugent, Robert
Project Start
1996-03-01
Project End
2002-02-28
Budget Start
2000-03-01
Budget End
2002-02-28
Support Year
5
Fiscal Year
2000
Total Cost
$283,850
Indirect Cost
Name
University of Miami School of Medicine
Department
Pediatrics
Type
Schools of Medicine
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146
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Tresoldi, Eleonora; Romiti, Maria Luisa; Boniotto, Michele et al. (2002) Prognostic value of the stromal cell-derived factor 1 3'A mutation in pediatric human immunodeficiency virus type 1 infection. J Infect Dis 185:696-700
Romiti, M L; Cancrini, C; Castelli-Gattinara, G et al. (2001) Kinetics of the T-cell receptor CD4 and CD8 V beta repertoire in HIV-1 vertically infected infants early treated with HAART. AIDS 15:2075-84
Laufer, M; Scott, G B (2000) Medical management of HIV disease in children. Pediatr Clin North Am 47:127-53