Abstract

We are conducting a randomized, double-blinded controlled trial among pregnant women in Cuernavaca, Mexico, to study the effect of daily supplementation with 200 mg docosahexaenoic acid (DHA) until delivery on a) birth outcomes b) maternal blood and breast milk DMA levels c) postnatal growth and development and d) infant DHA status. This is a collaborative effort among the Department of International Health, Emory University, and the Institute Nacional de Salud Publica in Mexico. The focus of this supplemental study is to evaluate the effect of DHA supplementation on infant immune function. We will analyze infant plasma and maternal breast milk samples currently being collected as part of the parent study protocol. DHA and other n-3 polyunsaturated fatty acids have been shown to modulate immune function in humans; however, few studies have addressed the independent effect of DHA. The central hypothesis of the supplemental study, that maternal DHA supplementation in pregnancy will modulate infant immune function, will be tested by measuring indicators of infant immune function including total immunoglobulin (Ig) concentrations and IgG sub-class concentrations in plasma, to be measured at birth (cord blood) and at 3 months. Antigen-specific antibody response after routine vaccination with 1 dose of diphtheria vaccine will be measured in infants at 3 months. We will measure breast milk IgA concentrations at 1 and 3 months post-partum. The sample size fixed by the parent study will be 116 mother-child pairs per group. The immunological assessments will be done at no cost to the study by the Centers for Disease Control, Atlanta. Data analysis will include group comparisons (intent-to-treat) after ensuring effectiveness of randomization. Adjusted comparisons (using co-variance analysis) will be made if control for potential confounders is required. To our knowledge, there are no studies that have evaluated the impact of maternal DHA supplementation in pregnancy on infant immune function. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
3R01HD043099-02S1
Application #
6967372
Study Section
Epidemiology of Chronic Diseases Study Section (ECD)
Program Officer
Grave, Gilman D
Project Start
2002-09-01
Project End
2008-01-31
Budget Start
2005-07-01
Budget End
2006-01-31
Support Year
2
Fiscal Year
2005
Total Cost
$65,757
Indirect Cost

  Institution

Name
Emory University
Department
Public Health & Prev Medicine
Type
Schools of Public Health
DUNS #
066469933
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

Gonzalez-Casanova, Ines; Stein, Aryeh D; Barraza-Villarreal, Albino et al. (2018) Prenatal exposure to environmental pollutants and child development trajectories through 7 years. Int J Hyg Environ Health 221:616-622
Kriss, Jennifer L; Ramakrishnan, Usha; Beauregard, Jennifer L et al. (2018) Yogurt consumption during pregnancy and preterm delivery in Mexican women: A prospective analysis of interaction with maternal overweight status. Matern Child Nutr 14:e12522
Gutierrez-Gomez, Yareni; Stein, Aryeh D; Ramakrishnan, Usha et al. (2017) Prenatal Docosahexaenoic Acid Supplementation Does Not Affect Nonfasting Serum Lipid and Glucose Concentrations of Offspring at 4 Years of Age in a Follow-Up of a Randomized Controlled Clinical Trial in Mexico. J Nutr 147:242-247
Gonzalez-Casanova, Ines; Stein, Aryeh D; Hao, Wei et al. (2015) Prenatal Supplementation with Docosahexaenoic Acid Has No Effect on Growth through 60 Months of Age. J Nutr 145:1330-4
Ramakrishnan, Usha; Stinger, Amanda; DiGirolamo, Ann M et al. (2015) Prenatal Docosahexaenoic Acid Supplementation and Offspring Development at 18 Months: Randomized Controlled Trial. PLoS One 10:e0120065
Ramirez-Silva, Ivonne; Rivera, Juan A; Trejo-Valdivia, Belem et al. (2015) Breastfeeding status at age 3 months is associated with adiposity and cardiometabolic markers at age 4 years in Mexican children. J Nutr 145:1295-302
Lee, Ho-Sun; Barraza-Villarreal, Albino; Biessy, Carine et al. (2014) Dietary supplementation with polyunsaturated fatty acid during pregnancy modulates DNA methylation at IGF2/H19 imprinted genes and growth of infants. Physiol Genomics 46:851-7
Lee, Ho-Sun; Hernandez-Vargas, Hector; Biessy, Carine et al. (2013) Reply to GC Burdge. Am J Clin Nutr 98:1595-6
Lee, Ho-Sun; Barraza-Villarreal, Albino; Hernandez-Vargas, Hector et al. (2013) Modulation of DNA methylation states and infant immune system by dietary supplementation with ?-3 PUFA during pregnancy in an intervention study. Am J Clin Nutr 98:480-7
Stein, Aryeh D; Wang, Meng; Rivera, Juan A et al. (2012) Auditory- and visual-evoked potentials in Mexican infants are not affected by maternal supplementation with 400 mg/d docosahexaenoic acid in the second half of pregnancy. J Nutr 142:1577-81

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