This competing renewal builds on successful completion of a double blind randomized controlled trial (RCT) of DHA supplementation during pregnancy in Cuernavaca, Mexico, and is a collaborative effort between the Hubert Department of Global Health, Emory University in Atlanta GA and the National Institute of Public Health (INSP) in Cuernavaca, Mexico. The overall objective is to extend to age 5 y our examination of the effects on the offspring of maternal supplementation during pregnancy with docosahexaenoic acid (DHA). Our central hypothesis is that DHA supplementation during pregnancy will improve early childhood development. In the original trial, 1094 pregnant women were randomized to receive daily a supplement of 400 mg of DHA or a placebo from 18-22 weeks of pregnancy. The 973 live births are currently being followed up to age 18 mos for measures of growth and development. We propose to study the birth cohort at age 5 y. We plan to collect a comprehensive set of measures of early childhood development (ECD) that are known to predict later intellectual functioning. The primary outcomes include global motor and mental development, executive functioning, specifically attention and memory, and inhibitory processes. Additional data on dietary intakes, health and exposure to early childhood stimulation both at the home and outside will also be obtained. All measurements will be obtained by a well trained and experienced field team;many team members participated in the parent study and have developed good rapport with the study population. We expect to recruit at least 85 percent of the eligible birth cohort and will have adequate power to detect effect sizes of 0.3 S.D. We will link these new data to our extraordinarily rich array of data that we collected prenatally, at birth, and at ages 1, 3, 6, 9, 12 and 18 mos. In addition to analyses that focus on the overall impact of supplementation with DHA, we will explore hypotheses concerning developmental trajectories. These data will also position us to continue to follow this cohort during their early school years.

Public Health Relevance

The objective of this competing renewal of grant HD43099 is to continue our examination of the effects of docosahexanoic acid (DHA) during pregnancy on child development at 5 years of age. This project builds on our successful completion of a large double blind randomized controlled trial of prenatal DHA supplementation in Cuernavaca, Mexico;we propose to resurvey the birth cohort (973 live births) at 4 years to maintain contact and at 5 years to obtain on a comprehensive set of measures of early childhood development (ECD). This renewal will strengthen our ongoing collaboration between Emory and National Institute of Public Health in Cuernavaca, Mexico and will provide the scientific evidence for making appropriate dietary recommendations for DHA during pregnancy to enhance human health and performance, especially educability.

National Institute of Health (NIH)
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Research Project (R01)
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Study Section
Neurological, Aging and Musculoskeletal Epidemiology (NAME)
Program Officer
Grave, Gilman D
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Emory University
Public Health & Prev Medicine
Schools of Public Health
United States
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Gonzalez-Casanova, Ines; Stein, Aryeh D; Barraza-Villarreal, Albino et al. (2018) Prenatal exposure to environmental pollutants and child development trajectories through 7 years. Int J Hyg Environ Health 221:616-622
Kriss, Jennifer L; Ramakrishnan, Usha; Beauregard, Jennifer L et al. (2018) Yogurt consumption during pregnancy and preterm delivery in Mexican women: A prospective analysis of interaction with maternal overweight status. Matern Child Nutr 14:e12522
Gutierrez-Gomez, Yareni; Stein, Aryeh D; Ramakrishnan, Usha et al. (2017) Prenatal Docosahexaenoic Acid Supplementation Does Not Affect Nonfasting Serum Lipid and Glucose Concentrations of Offspring at 4 Years of Age in a Follow-Up of a Randomized Controlled Clinical Trial in Mexico. J Nutr 147:242-247
Gonzalez-Casanova, Ines; Stein, Aryeh D; Hao, Wei et al. (2015) Prenatal Supplementation with Docosahexaenoic Acid Has No Effect on Growth through 60 Months of Age. J Nutr 145:1330-4
Ramakrishnan, Usha; Stinger, Amanda; DiGirolamo, Ann M et al. (2015) Prenatal Docosahexaenoic Acid Supplementation and Offspring Development at 18 Months: Randomized Controlled Trial. PLoS One 10:e0120065
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Lee, Ho-Sun; Hernandez-Vargas, Hector; Biessy, Carine et al. (2013) Reply to GC Burdge. Am J Clin Nutr 98:1595-6
Lee, Ho-Sun; Barraza-Villarreal, Albino; Hernandez-Vargas, Hector et al. (2013) Modulation of DNA methylation states and infant immune system by dietary supplementation with ?-3 PUFA during pregnancy in an intervention study. Am J Clin Nutr 98:480-7
Stein, Aryeh D; Wang, Meng; Rivera, Juan A et al. (2012) Auditory- and visual-evoked potentials in Mexican infants are not affected by maternal supplementation with 400 mg/d docosahexaenoic acid in the second half of pregnancy. J Nutr 142:1577-81

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