Abstract

Chromosome-specific DNA libraries have been of considerable utility in mapping the human genome. However, conventionally sorted libraries are expensive to produce and are frequently contaminated with other sequences. We propose to use a new methodology, denoted coincidence cloning, to produce chromosome specific libraries from a somatic cell hybrid that contains part of the short arm of human chromosome 4 and most of human chromosome 5. We shall assess the efficiency of four modifications of this technology in constructing such a library and shall characterize the sequence content and representation of these libraries. Mouse chromosomes to date have been refractory to currently available sorting techniques. We shall therefore construct a chromosome specific library from a somatic cell hybrid that contains the tip of mouse chromosome 16 and the majority of mouse chromosome 2. Linking libraries that contain rare cutting restriction enzyme sites will also be constructed from both the human and mouse chromosomes 2. Linking clones will then be used to construct a physical map of overlapping fragments and to derive a genetic map of these markers on the two chromosomes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
5R01HG000368-04
Application #
3333534
Study Section
Special Emphasis Panel (SRC)
Project Start
1988-09-30
Project End
1992-06-30
Budget Start
1991-09-01
Budget End
1992-06-30
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Genelabs Technologies, Inc.
Department
Type
DUNS #
City
Redwood City
State
CA
Country
United States
Zip Code
94063

  Publications

Boyadjiev, S A; Chowdry, A B; Shapiro, R E et al. (2002) Physical map of the chromosome 6q22 region containing the oculodentodigital dysplasia locus: analysis of thirteen candidate genes and identification of novel ESTs and DNA polymorphisms. Cytogenet Genome Res 98:29-37
Rojas, K; Serrano de la Pena, L; Gallardo, T et al. (1999) Physical map and characterization of transcripts in the candidate interval for familial chondrocalcinosis at chromosome 5p15.1. Genomics 62:177-83
Hastbacka, J; Kerrebrock, A; Mokkala, K et al. (1999) Identification of the Finnish founder mutation for diastrophic dysplasia (DTD). Eur J Hum Genet 7:664-70
Bardien-Kruger, S; Greenberg, J; Tubb, B et al. (1999) Refinement of the RP17 locus for autosomal dominant retinitis pigmentosa, construction of a YAC contig and investigation of the candidate gene retinal fascin. Eur J Hum Genet 7:332-8
Simmons, A D; Overhauser, J; Lovett, M (1997) Isolation of cDNAs from the Cri-du-chat critical region by direct screening of a chromosome 5-specific cDNA library. Genome Res 7:118-27
Touchman, J W; Bouffard, G G; Weintraub, L A et al. (1997) 2006 expressed-sequence tags derived from human chromosome 7-enriched cDNA libraries. Genome Res 7:281-92
Wise, C A; Clines, G A; Massa, H et al. (1997) Identification and localization of the gene for EXTL, a third member of the multiple exostoses gene family. Genome Res 7:10-6
Clines, G A; Ashley, J A; Shah, S et al. (1997) The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans. Genome Res 7:359-67
Stickens, D; Clines, G; Burbee, D et al. (1996) The EXT2 multiple exostoses gene defines a family of putative tumour suppressor genes. Nat Genet 14:25-32
Guimera, J; Casas, C; Pucharcos, C et al. (1996) A human homologue of Drosophila minibrain (MNB) is expressed in the neuronal regions affected in Down syndrome and maps to the critical region. Hum Mol Genet 5:1305-10

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