The first goal of this project is to assess the feasibility of using a newly developed, rapid, high resolution, visual mapping procedure for the mapping of yeast artificial chromosomes. The assembly of maps from yeast artificial chromosomes for the human genome will facilitate identification of genetic disorders and the gene responsible. The second goal is to improve this new mapping procedure by developing a multi-color multi-probe strategy and developing computer software capable of analyzing the visual images that are generated. These improvements will allow faster mapping as well as provide the ability to rapidly and precisely detect gene rearrangements involved in human disorders. The new mapping technology that will be used involves the stretching of human genomic DNA on a slide and hybridizing fluorescent DNA probes to the extended DNA strands. The result is a multi-color string as viewed through a fluorescence microscope and represent a map of the DNA region of interest. Resolution as low as .4 Kb has been obtained. Yeast artificial chromosomes from human chromosome 3 will be studied with this technology.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
1R01HG000975-01
Application #
2209210
Study Section
Genome Study Section (GNM)
Project Start
1993-12-01
Project End
1996-11-30
Budget Start
1993-12-01
Budget End
1994-11-30
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Ctrc Research Foundation
Department
Type
DUNS #
City
San Antonio
State
TX
Country
United States
Zip Code
78229
Windle, B; Silvas, E; Parra, I (1995) High resolution microscopic mapping of DNA using multi-color fluorescent hybridization. Electrophoresis 16:273-8