Abstract

This grant represents in effect a competitive renewal for 2 grants, HL-18918 (Characterization of Lung Elastin) and HL-15965 (Elastin metabolism in Atherosclerosis).
The specific aims of both grants have been combined and are: 1) to isolate and characterize elastin fiber precursors from lung and aorta sources, 2) quantify changes in elastin synthesis and accumulation (deposition) during lund and aorta development, 3) identify factors that influence elastin metabolism, and 4) identify or clarify post-translation steps important to elastic fiber deposition. The methodologies will involve whole animal, tissue culture, and cell-free translation studies dealing eith elastin biosynthesis in relationship to development, diet, endocrine, and disease model relationships. It is the hope that the findings will have application to concepts inportant to a better understanding of elastin's role in developmental emphysema, other forms of chronic obstructive lung disease and vessel wall development in health and disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL015965-13
Application #
3335077
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1979-05-01
Project End
1987-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Type
Earth Sciences/Resources
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Dodge, D E; Plopper, C G; Rucker, R B (1994) Regulation of Clara cell 10 kD protein secretion by pilocarpine: quantitative comparison of nonciliated cells in rat bronchi and bronchioles based on laser scanning confocal microscopy. Am J Respir Cell Mol Biol 10:259-70
Dodge, D E; Rucker, R B; Pinkerton, K E et al. (1994) Dose-dependent tolerance to ozone. III. Elevation of intracellular Clara cell 10-kDa protein in central acini of rats exposed for 20 months. Toxicol Appl Pharmacol 127:109-23
Dodge, D E; Rucker, R B; Singh, G et al. (1993) Quantitative comparison of intracellular concentration and volume of Clara cell 10 KD protein in rat bronchi and bronchioles based on laser scanning confocal microscopy. J Histochem Cytochem 41:1171-83
Rucker, R B; McGee, C (1993) Chemical modifications of proteins in vivo: selected examples important to cellular regulation. J Nutr 123:977-90
Reiser, K; McCormick, R J; Rucker, R B (1992) Enzymatic and nonenzymatic cross-linking of collagen and elastin. FASEB J 6:2439-49
Romero-Chapman, N; Lee, J; Tinker, D et al. (1991) Purification, properties and influence of dietary copper on accumulation and functional activity of lysyl oxidase in rat skin. Biochem J 275 ( Pt 3):657-62
Sharrow, L; Tinker, D; Davidson, J M et al. (1989) Accumulation and regulation of elastin in the rat uterus. Proc Soc Exp Biol Med 192:121-6
Killgore, J; Smidt, C; Duich, L et al. (1989) Nutritional importance of pyrroloquinoline quinone. Science 245:850-2
Iredale, R B; Eccleston-Joyner, C A; Rucker, R B et al. (1989) Ontogenic development of the elastic component of the aortic wall in spontaneously hypertensive rats. Clin Exp Hypertens A 11:173-87
Chung, K; Romero, N; Tinker, D et al. (1988) Role of copper in the regulation and accumulation of superoxide dismutase and metallothionein in rat liver. J Nutr 118:859-64

Showing the most recent 10 out of 19 publications